PXD056869

PXD056869 is an original dataset announced via ProteomeXchange.

Title	Molecular characterization of Calu-3 cells from submerged to Air-liquid interface to model lung infections
Description	Air-liquid interface (ALI) model using the immortalized Calu-3 cells has been used to model lung diseases. From submerged to ALI culture condition, Calu-3 cells polarize and change to a ciliary mucus-producing cell. Polarized Calu-3 molecular and phenotypic similarity with primary cells has been proven; however, no studies have been focused on the pathways differentially expressed in ALI vs. submerged conditions. In this study, we profiled the proteome and transcriptome of Calu-3 cells from submerged (non-polarized, NP) to ALI (polarized, P) culture conditions and in the omics data we observed an increase in cell replication in the non-polarized condition while polarized Calu-3 cells presented higher activation of cellular energy production, protein maturation and recycle and expression of immune active molecules. Moreover, the omics findings showed upregulation of different biological processes related to protein quality control system and antigen processing presentation in polarized Calu-3 cells. Immunoblot and fluorescence microscopy confirmed increased expression of bronchial epithelium integrity components like mucus and tight junctions in polarized Calu-3 and revealed a characteristic protein expression and cellular organization found in normal lung epithelial tissue. Furthermore, SARS-CoV-2 infection of polarized cells revealed higher cell death due to higher viral entry associated with higher apical membrane expression of ACE2 receptor. The differences observed in this study give us a better understanding of how ALI can mimic the human bronchial-epithelial cells and its applications in different contexts of lung diseases.
HostingRepository	PRIDE
AnnounceDate	2026-02-23
AnnouncementXML	Submission_2026-02-23_03:02:56.586.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Deivid Martins Santos
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-16 12:37:02	ID requested
⏵ 1	2026-02-23 03:02:57	announced

10.1021/acs.jproteome.4c00975;

Martins Santos D, de Souza EE, Macedo-da-Silva J, Oba-Shinjo SM, Angeli CB, de Morais Gomes V, Mule SN, Trajano LAM, Souza-Silva GA, Boscardin SB, Durigon EL, Jaeger RG, Freitas VM, Wrenger C, Larsen MR, Rosa-Fernandes L, Nagashi Marie SK, Palmisano G, Molecular Characterization of Calu-3 Cells from Submerged to Air-Liquid Interface to Model Lung Infections. J Proteome Res, 25(2):562-577(2026) [pubmed]

submitter keyword: Calu-3, Proteomics,ALI, Polarization

Giuseppe Palmisano
contact affiliation	Glycoproteomics Lab, Parasitology department, Institute of Biomedical Sciences of University of São Paulo, Brazil.
contact email	palmisano.gp@usp.br
lab head
Deivid Martins Santos
contact affiliation	University of São Paulo
contact email	deivid.martinss98@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD056869

PRIDE project URI

• PRIDE
  1. PXD056869
     1. Label: PRIDE project
     2. Name: Molecular characterization of Calu-3 cells from submerged to Air-liquid interface to model lung infections