PXD056865

PXD056865 is an original dataset announced via ProteomeXchange.

Title	Affinity-based chemoproteomic profiling of UNC0638 and UNC0737 in MCF7 and PBMC lysates identifies CHKA as a shared off-target.
Description	To identify the molecular target(s) and elucidate the mechanism of action of UNC0638 and UNC0737, affinity-based chemoproteomics was performed. UNC0965, a biotinylated analogue of UNC0638, was immobilised on streptavidin-coated sepharose beads to enrich binding proteins from whole MCF7 or PBMC cell lysates. UNC0636 or UNC0737 were added as competitors in control experiments to identify specific binders.
HostingRepository	PRIDE
AnnounceDate	2025-01-29
AnnouncementXML	Submission_2025-01-29_04:44:42.263.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ludwig Bauer
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-16 10:52:19	ID requested
1	2025-01-27 12:08:37	announced
⏵ 2	2025-01-29 04:44:43	announced	2025-01-29: Updated project metadata.

10.1002/anie.202420149;

Bauer LG, Ward JA, D, í, az-S, á, ez L, Sundstr, ö, m Y, Tolvanen T, Alarc, ó, n Barrera JC, Kostidis S, Rogers CM, Panagakou I, Singh U, Rothweiler EM, Gonzalez Orta A, Kaniskan H Ü, Hu J, Jin J, Sievers S, Waldmann H, Giera M, Sundstr, ö, m M, Berg L, Huber KVM, Phenomics-Based Discovery of Novel Orthosteric Choline Kinase Inhibitors. Angew Chem Int Ed Engl, 64(7):e202420149(2025) [pubmed]

submitter keyword: Chemical Proteomics

Kilian Huber
contact affiliation	Centre for Medicines Discovery & Target Discovery Institute, Nuffield Department of Medicine, University of Oxford, Oxford, UK
contact email	kilian.huber@cmd.ox.ac.uk
lab head
Ludwig Bauer
contact affiliation	University of Oxford
contact email	ludwig.bauer@st-hughs.ox.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/01/PXD056865

PRIDE project URI

• PRIDE
  1. PXD056865
     1. Label: PRIDE project
     2. Name: Affinity-based chemoproteomic profiling of UNC0638 and UNC0737 in MCF7 and PBMC lysates identifies CHKA as a shared off-target.