PXD056835
PXD056835 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomic analysis of B cells in peripheral lymphatic system reveals the dynamics during the systemic lupus erythematosus progression |
| Description | In this study, we conducted a comprehensive proteomic analysis of B cells from spleen, mesenteric lymph nodes (mLN), and peripheral blood mononuclear cells (PBMCs) in a time-course model of systemic lupus erythematosus (SLE) using female MRL/lpr mice. By combining fluorescence-activated cell sorting (FACS) and 4D-Data-Independent Acquisition (4D-DIA) mass spectrometry, we quantified nearly 8,000 proteins, identifying significant temporal and tissue-specific proteomic changes during SLE progression. PBMC-derived B cells exhibited early proteomic alterations by week 9, while spleen-derived B cells showed similar changes by week 12. We identified key regulatory proteins, including BAFF, BAFFR, and NFKB2, involved in B cell survival and activation, as well as novel markers such as CD11c and CD117, which have previously been associated with other immune cells. The study highlights the dynamic reprogramming of B cell proteomes across different tissues, with distinct contributions to SLE pathogenesis, providing valuable insights into the molecular mechanisms underlying B cell dysregulation in lupus. These findings offer potential therapeutic targets and biomarkers for SLE. |
| HostingRepository | iProX |
| AnnounceDate | 2024-10-15 |
| AnnouncementXML | Submission_2024-10-30_18:27:13.097.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Liming Sun |
| SpeciesList | scientific name: Mus musculus; NCBI TaxID: 10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | timsTOF Pro |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-10-15 21:54:53 | ID requested | |
| ⏵ 1 | 2024-10-30 18:27:13 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: SLE, B cells, Proteomics |
Contact List
| Rong Zeng | |
|---|---|
| contact affiliation | Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, Shanghai, China 200031 |
| contact email | zr@sibcb.ac.cn |
| lab head | |
| Liming Sun | |
| contact affiliation | University of Chinese Academy of Sciences |
| contact email | sunlm@ucas.ac.cn |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
to receive all new ProteomeXchange dataset release announcements!
