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PXD056835

PXD056835 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic analysis of B cells in peripheral lymphatic system reveals the dynamics during the systemic lupus erythematosus progression
DescriptionIn this study, we conducted a comprehensive proteomic analysis of B cells from spleen, mesenteric lymph nodes (mLN), and peripheral blood mononuclear cells (PBMCs) in a time-course model of systemic lupus erythematosus (SLE) using female MRL/lpr mice. By combining fluorescence-activated cell sorting (FACS) and 4D-Data-Independent Acquisition (4D-DIA) mass spectrometry, we quantified nearly 8,000 proteins, identifying significant temporal and tissue-specific proteomic changes during SLE progression. PBMC-derived B cells exhibited early proteomic alterations by week 9, while spleen-derived B cells showed similar changes by week 12. We identified key regulatory proteins, including BAFF, BAFFR, and NFKB2, involved in B cell survival and activation, as well as novel markers such as CD11c and CD117, which have previously been associated with other immune cells. The study highlights the dynamic reprogramming of B cell proteomes across different tissues, with distinct contributions to SLE pathogenesis, providing valuable insights into the molecular mechanisms underlying B cell dysregulation in lupus. These findings offer potential therapeutic targets and biomarkers for SLE.
HostingRepositoryiProX
AnnounceDate2024-10-15
AnnouncementXMLSubmission_2024-10-30_18:27:13.097.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLiming Sun
SpeciesList scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationListNo PTMs are included in the dataset
InstrumenttimsTOF Pro
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-10-15 21:54:53ID requested
12024-10-30 18:27:13announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: SLE, B cells, Proteomics
Contact List
Rong Zeng
contact affiliationCenter for Excellence in Molecular Cell Science, Chinese Academy of Sciences, Shanghai, China 200031
contact emailzr@sibcb.ac.cn
lab head
Liming Sun
contact affiliationUniversity of Chinese Academy of Sciences
contact emailsunlm@ucas.ac.cn
dataset submitter
Full Dataset Link List
iProX dataset URI