PXD056818

PXD056818 is an original dataset announced via ProteomeXchange.

Title	A novel non-galactosylated tolerogenic antigen-specific vaccine protects against arthritis via VISTA-expressing regulatory T cells in DRB1*04:01 mice
Description	Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by joint inflammation, cartilage damage and bone erosion. Despite improvements with the introduction of biological disease-modifying anti-rheumatic drugs (DMARDs), RA remains incurable. Advancements in peptide-based vaccination may open new avenues for treating autoimmune diseases by inducing immune tolerance while maintaining normal immune function. We have demonstrated a potential vaccine against RA, made by mouse major histocompatibility complex class II (Aq) protein bounding the immunodominant type II collagen peptide COL2259-273, which needed to be galactosylated at position 264. To translate the vaccine to humans and to further enhance and simplify this vaccine, we modified the amino acid residue at position 265 and conjugated it with the human DRB1*04:01 molecule. Remarkably, this modified vaccine proved effective in DRB1*04:01-expressing mice without the need for galactosylation at position 264. The vaccine exerts its protective effect through the activation and expansion of VISTA-positive nonconventional regulatory T cells, leading to a potent dominant suppressive response that prevents arthritis development in mice. The new modified vaccine for RA, offers a novel therapeutic approach with potential clinical implications for human autoimmune diseases.
HostingRepository	PRIDE
AnnounceDate	2026-06-29
AnnouncementXML	Submission_2026-06-29_03:47:35.140.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christian Beusch
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-15 08:15:20	ID requested
⏵ 1	2026-06-29 03:47:36	announced

10.1016/j.ymthe.2025.04.034;

Romero-Castillo L, Pandey RK, Xu B, Beusch CM, Oliveira-Coelho A, Zeqiraj K, Svensson C, Xu Z, Luo H, Sareila O, Sabatier P, Ge C, Cheng L, Urbonaviciute V, Kr, ä, mer A, Lindgren C, Haag S, Viljanen J, Zubarev RA, Kihlberg J, Linusson A, Burkhardt H, Holmdahl R, 04:01 mice. Mol Ther, 33(8):3528-3545(2025) [pubmed]

submitter keyword: vaccine, arthritis, mice, immune cells

Rikard Holmdahl
contact affiliation	Medical Inflammation Research, Division of Immunology, Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden
contact email	Rikard.holmdahl@ki.se
lab head
Christian Beusch
contact affiliation	Emory University
contact email	christian.beusch@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD056818
     1. Label: PRIDE project
     2. Name: A novel non-galactosylated tolerogenic antigen-specific vaccine protects against arthritis via VISTA-expressing regulatory T cells in DRB1*04:01 mice