PXD056815

PXD056815 is an original dataset announced via ProteomeXchange.

Title	Melatonin confers saline-alkali tolerance by relieving nitrosative damage and S-nitrosylation of plasma membrane H+-ATPase 2 in tomato
Description	Soil salination and alkalization are global problems impairing plant survival by disrupting REDOX homeostasis. Whether melatonin regulates REDOX homeostasis at nitrosative level, and thus affects plant saline-alkali tolerance remains unknown. In saline-alkali stress, excess nitric oxide (NO) causes nitrosative damage in tomato roots. This NO can be degraded by S-nitrosoglutathione reductase (GSNOR), or stimulates caffeic acid O-methyltransferase (COMT) transcript for melatonin synthesis. Melatonin further feedback scavenges excess NO to alleviate nitrosative damage at the whole protein level, indicating by proteome S-nitrosylation. We target plasma membrane H+-ATPase 2 (HA2) and highlight that HA2 is S-nitrosylated at Cys206 in saline-alkali stress, reducing HA activity, H+ efflux, and tolerance by impairing its interaction with 14-3-3 protein 1 (TFT1). In agreement with these observations, COMT-mediated melatonin relieves the HA2 S-nitrosylation to recover its function and saline-alkali tolerance. Therefore, we propose NO and melatonin as a pair of REDOX switches to control HA2 S-nitrosylation and saline-alkali tolerance. Under natural saline-alkali conditions, tomato productivity can be improved by grafting with COMT-, GSNOR-, HA2-overexpression rootstocks or by genetic engineering non-nitrosylated HA2C206S mutants. Using melatonin-NO-HA2 module as a case, this study illuminates a novel molecular function of melatonin and relevant genetic engineering strategies in future agriculture.
HostingRepository	PRIDE
AnnounceDate	2025-04-28
AnnouncementXML	Submission_2025-04-28_07:27:11.286.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	guochen qin
SpeciesList	scientific name: 'Solanum lycopersicum' phytoplasma; NCBI TaxID: 1334098;
ModificationList	S-nitrosyl-L-cysteine
Instrument	orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-15 08:02:59	ID requested
⏵ 1	2025-04-28 07:27:12	announced

Wei JW, Liu M, Zhao D, Du P, Yan L, Liu D, Shi Q, Yang C, Qin G, Gong B, Melatonin confers saline-alkali tolerance in tomato by alleviating nitrosative damage and S-nitrosylation of H+-ATPase 2. Plant Cell, 37(2):(2025) [pubmed]

10.1093/plcell/koaf035;

submitter keyword: Melatonin

Nitric oxide

Plasma membrane H+-ATPase 2

Saline-alkali stress

S-nitrosylation

Tomato

guochen qin
contact affiliation	Peking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agricultural Sciences at Weifang, Weifang, 261000, China
contact email	guochen.qin@pku-iaas.edu.cn
lab head
guochen qin
contact affiliation	Peking University Institute of Advanced Agricultural Sciences
contact email	guochen.qin@pku-iaas.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD056815
     1. Label: PRIDE project
     2. Name: Melatonin confers saline-alkali tolerance by relieving nitrosative damage and S-nitrosylation of plasma membrane H+-ATPase 2 in tomato