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PXD056752

PXD056752 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA nuclear pore-anchored condensate enables germ granule organisation and transgenerational epigenetic inheritance
DescriptionBiomolecular condensates, such as stress and germ granules, often contain sub-compartments. For instance, the Caenorhabditis elegans germ granule, which localizes near the outer nuclear membrane of germ cell nuclei, is composed of at least four ordered compartments, each housing distinct sets of proteins and RNAs. How these compartments form and why they are spatially ordered remains poorly understood. Here, we show that the conserved RNA helicase DDX-19 defines another compartment of the larger C. elegans germ granule, which we term the D compartment. The D compartment exhibits properties of a liquid condensate and forms between the outer nuclear pore filament and other compartments of the germ granule. Two nuclear pore proteins, NPP-14 and GLEL-1, are required for its formation, suggesting that the D compartment localizes adjacent to the outer nuclear membrane via interactions with the nuclear pore. The loss of DDX-19, NPP-14, or GLEL-1 leads to functional defects, including aberrant formation of the other four germ granule compartments, a loss of germline immortality, and dysregulation of small RNA-based transgenerational epigenetic inheritance programs. Hence, we propose that a function of the D compartment is to anchor larger germ granules to nuclear pores, enabling germ granule compartmentalization and promoting transgenerational RNA surveillance.
HostingRepositoryPRIDE
AnnounceDate2025-02-18
AnnouncementXMLSubmission_2025-02-18_07:08:24.130.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD056752
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterBoyuan Deng
SpeciesList scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-10-12 00:54:49ID requested
12025-02-08 19:15:27announced
22025-02-18 07:08:24announced2025-02-18: Updated project metadata.
Publication List
10.6019/PXD056752;
Keyword List
submitter keyword: transgenerational epigenetic inheritance, DDX-19,condensates,C.elegans
Contact List
Gang Wan
contact affiliationGuangdong Province Key Laboratory of Pharmaceutical Functional Genes, MOE Key Laboratory of Gene Function and Regulation, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, China
contact emailwangang5@mail.sysu.edu.cn
lab head
Boyuan Deng
contact affiliationSchool of Life Sciences, Sun Yat-sen University, Guangzhou, China
contact emaildengby26@mail2.sysu.edu.cn
dataset submitter
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Dataset FTP location
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