PXD056752 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A nuclear pore-anchored condensate enables germ granule organisation and transgenerational epigenetic inheritance |
Description | Biomolecular condensates, such as stress and germ granules, often contain sub-compartments. For instance, the Caenorhabditis elegans germ granule, which localizes near the outer nuclear membrane of germ cell nuclei, is composed of at least four ordered compartments, each housing distinct sets of proteins and RNAs. How these compartments form and why they are spatially ordered remains poorly understood. Here, we show that the conserved RNA helicase DDX-19 defines another compartment of the larger C. elegans germ granule, which we term the D compartment. The D compartment exhibits properties of a liquid condensate and forms between the outer nuclear pore filament and other compartments of the germ granule. Two nuclear pore proteins, NPP-14 and GLEL-1, are required for its formation, suggesting that the D compartment localizes adjacent to the outer nuclear membrane via interactions with the nuclear pore. The loss of DDX-19, NPP-14, or GLEL-1 leads to functional defects, including aberrant formation of the other four germ granule compartments, a loss of germline immortality, and dysregulation of small RNA-based transgenerational epigenetic inheritance programs. Hence, we propose that a function of the D compartment is to anchor larger germ granules to nuclear pores, enabling germ granule compartmentalization and promoting transgenerational RNA surveillance. |
HostingRepository | PRIDE |
AnnounceDate | 2025-02-18 |
AnnouncementXML | Submission_2025-02-18_07:08:24.130.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD056752 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Boyuan Deng |
SpeciesList | scientific name: Caenorhabditis elegans; NCBI TaxID: 6239; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-10-12 00:54:49 | ID requested | |
1 | 2025-02-08 19:15:27 | announced | |
⏵ 2 | 2025-02-18 07:08:24 | announced | 2025-02-18: Updated project metadata. |
Publication List
Keyword List
submitter keyword: transgenerational epigenetic inheritance, DDX-19,condensates,C.elegans |
Contact List
Gang Wan |
contact affiliation | Guangdong Province Key Laboratory of Pharmaceutical Functional Genes, MOE Key Laboratory of Gene Function and Regulation, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, China |
contact email | wangang5@mail.sysu.edu.cn |
lab head | |
Boyuan Deng |
contact affiliation | School of Life Sciences, Sun Yat-sen University, Guangzhou, China |
contact email | dengby26@mail2.sysu.edu.cn |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD056752
- Label: PRIDE project
- Name: A nuclear pore-anchored condensate enables germ granule organisation and transgenerational epigenetic inheritance