PXD056752

PXD056752 is an original dataset announced via ProteomeXchange.

Title	A nuclear pore-anchored condensate enables germ granule organisation and transgenerational epigenetic inheritance
Description	Biomolecular condensates, such as stress and germ granules, often contain sub-compartments. For instance, the Caenorhabditis elegans germ granule, which localizes near the outer nuclear membrane of germ cell nuclei, is composed of at least four ordered compartments, each housing distinct sets of proteins and RNAs. How these compartments form and why they are spatially ordered remains poorly understood. Here, we show that the conserved RNA helicase DDX-19 defines another compartment of the larger C. elegans germ granule, which we term the D compartment. The D compartment exhibits properties of a liquid condensate and forms between the outer nuclear pore filament and other compartments of the germ granule. Two nuclear pore proteins, NPP-14 and GLEL-1, are required for its formation, suggesting that the D compartment localizes adjacent to the outer nuclear membrane via interactions with the nuclear pore. The loss of DDX-19, NPP-14, or GLEL-1 leads to functional defects, including aberrant formation of the other four germ granule compartments, a loss of germline immortality, and dysregulation of small RNA-based transgenerational epigenetic inheritance programs. Hence, we propose that a function of the D compartment is to anchor larger germ granules to nuclear pores, enabling germ granule compartmentalization and promoting transgenerational RNA surveillance.
HostingRepository	PRIDE
AnnounceDate	2025-02-18
AnnouncementXML	Submission_2025-02-18_07:08:24.130.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD056752
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Boyuan Deng
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-12 00:54:49	ID requested
1	2025-02-08 19:15:27	announced
⏵ 2	2025-02-18 07:08:24	announced	2025-02-18: Updated project metadata.

10.6019/PXD056752;

submitter keyword: transgenerational epigenetic inheritance, DDX-19,condensates,C.elegans

Gang Wan
contact affiliation	Guangdong Province Key Laboratory of Pharmaceutical Functional Genes, MOE Key Laboratory of Gene Function and Regulation, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, China
contact email	wangang5@mail.sysu.edu.cn
lab head
Boyuan Deng
contact affiliation	School of Life Sciences, Sun Yat-sen University, Guangzhou, China
contact email	dengby26@mail2.sysu.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/02/PXD056752

PRIDE project URI

• PRIDE
  1. PXD056752
     1. Label: PRIDE project
     2. Name: A nuclear pore-anchored condensate enables germ granule organisation and transgenerational epigenetic inheritance