PXD056736

PXD056736 is an original dataset announced via ProteomeXchange.

Title	Therapeutic hyperthermia promotes lipid export and HSP70/90 during machine perfusion of human livers
Description	Liver transplantation is the only curative option for end-stage liver disease. Donor shortages necessitate the use of higher risk donor livers, including fatty livers, which are more susceptible to ischemia reperfusion injury. Machine perfusion has improved graft utilization and is typically performed at hypothermic (8-12oC), or normothermic (35-37oC) temperatures. Here we studied the impact of mild hyperthermia (40oC) as a therapeutic intervention for fatty livers using in-depth proteomic and lipoprotein profiling of whole organ perfusion and precision cut liver slices. We observed proteomic changes with metabolic alterations over time, evidenced by a significant increase in lipid export in whole organ perfusions. Furthermore, PCLS showed significant upregulation of metabolic processes and heat shock protein response after 24 hours of hyperthermia. Machine perfusion under hyperthermic conditions may be a potential strategy to improve the utilization of fatty liver grafts, ultimately expanding the donor pool and improving transplant outcomes.
HostingRepository	PRIDE
AnnounceDate	2025-05-26
AnnouncementXML	Submission_2025-05-25_17:35:15.732.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Adam Thorne
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-11 11:09:59	ID requested
⏵ 1	2025-05-25 17:35:16	announced

10.14814/phy2.70348;

Thorne AM, Geng Y, Lantinga VA, Smit M, Kuivenhoven JA, Porte RJ, Kuipers F, Olinga P, Wolters JC, de Meijer VE, Therapeutic hyperthermia promotes lipid export and HSP70/90 during machine perfusion of human livers. Physiol Rep, 13(9):e70348(2025) [pubmed]

submitter keyword: proteomics, transplant, hyperthermia,Liver, machine perfusion

Vincent de Meijer
contact affiliation	University Medical Center Groningen and University of Groningen
contact email	v.e.de.meijer@umcg.nl
lab head
Adam Thorne
contact affiliation	University Medical Center Groningen
contact email	a.m.thorne@umcg.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD056736

PRIDE project URI

• PRIDE
  1. PXD056736
     1. Label: PRIDE project
     2. Name: Therapeutic hyperthermia promotes lipid export and HSP70/90 during machine perfusion of human livers