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PXD056736

PXD056736 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTherapeutic hyperthermia promotes lipid export and HSP70/90 during machine perfusion of human livers
DescriptionLiver transplantation is the only curative option for end-stage liver disease. Donor shortages necessitate the use of higher risk donor livers, including fatty livers, which are more susceptible to ischemia reperfusion injury. Machine perfusion has improved graft utilization and is typically performed at hypothermic (8-12oC), or normothermic (35-37oC) temperatures. Here we studied the impact of mild hyperthermia (40oC) as a therapeutic intervention for fatty livers using in-depth proteomic and lipoprotein profiling of whole organ perfusion and precision cut liver slices. We observed proteomic changes with metabolic alterations over time, evidenced by a significant increase in lipid export in whole organ perfusions. Furthermore, PCLS showed significant upregulation of metabolic processes and heat shock protein response after 24 hours of hyperthermia. Machine perfusion under hyperthermic conditions may be a potential strategy to improve the utilization of fatty liver grafts, ultimately expanding the donor pool and improving transplant outcomes.
HostingRepositoryPRIDE
AnnounceDate2025-05-26
AnnouncementXMLSubmission_2025-05-25_17:35:15.732.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAdam Thorne
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-10-11 11:09:59ID requested
12025-05-25 17:35:16announced
Publication List
10.14814/phy2.70348;
Thorne AM, Geng Y, Lantinga VA, Smit M, Kuivenhoven JA, Porte RJ, Kuipers F, Olinga P, Wolters JC, de Meijer VE, Therapeutic hyperthermia promotes lipid export and HSP70/90 during machine perfusion of human livers. Physiol Rep, 13(9):e70348(2025) [pubmed]
Keyword List
submitter keyword: proteomics, transplant, hyperthermia,Liver, machine perfusion
Contact List
Vincent de Meijer
contact affiliationUniversity Medical Center Groningen and University of Groningen
contact emailv.e.de.meijer@umcg.nl
lab head
Adam Thorne
contact affiliationUniversity Medical Center Groningen
contact emaila.m.thorne@umcg.nl
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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