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PXD056728

PXD056728 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHeteroresistance in Enterobacter cloacae complex caused by cell-to-cell variation in transient gene duplication-amplification events
DescriptionHeteroresistance in bacteria describes a subpopulational phenomenon of transient antibiotic resistance variation among cells of a generally susceptible population. Here, we investigated the molecular mechanisms and phenotypic characteristics underlying heteroresistance to ceftazidime (CAZ) in a clinical Enterobacter cloacae complex strain (ECC). We identified a plasmid-borne gene duplication-amplification (GDA) event of a region harboring an ampC gene encoding a β-lactamase blaDHA-1 as the key determinant of heteroresistance. Individual colonies exhibited variations in the copy number of the genes resulting in resistance level variation which correlated with growth onset (lag times) and growth rates in the presence of CAZ, analysed in linear models. GDA copy number heterogeneity occurred within single resistant colonies, demonstrating heterogeneity of GDA on the single-cell level. The interdependence between GDA, lag time and antibiotic treatment and the strong plasticity underlying heteroresistance underlines the high risk for misdetection of antimicrobial heteroresistance and subsequent treatment failure.
HostingRepositoryPRIDE
AnnounceDate2024-10-17
AnnouncementXMLSubmission_2024-10-17_01:07:15.317.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterBenno Kuropka
SpeciesList scientific name: Enterobacter cloacae; NCBI TaxID: 550;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-10-11 06:32:57ID requested
12024-10-17 01:07:15announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: LC-MS/MS
Contact List
Marcus Fulde
contact affiliationDepartment of Veterinary Medicine, Institute of Microbiology and Epizootics, Centre for Infection Medicine, Freie Universität Berlin, Berlin, Germany
contact emailmarcus.fulde@fu-berlin.de
lab head
Benno Kuropka
contact affiliationFreie Universität Berlin, Institute of Chemistry and Biochemistry, Laboratory of Protein Biochemistry, Thielallee 63, D-14195, Berlin, Germany
contact emailkuropka@zedat.fu-berlin.de
dataset submitter
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Dataset FTP location
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