PXD056728

PXD056728 is an original dataset announced via ProteomeXchange.

Title	Heteroresistance in Enterobacter cloacae complex caused by cell-to-cell variation in transient gene duplication-amplification events
Description	Heteroresistance in bacteria describes a subpopulational phenomenon of transient antibiotic resistance variation among cells of a generally susceptible population. Here, we investigated the molecular mechanisms and phenotypic characteristics underlying heteroresistance to ceftazidime (CAZ) in a clinical Enterobacter cloacae complex strain (ECC). We identified a plasmid-borne gene duplication-amplification (GDA) event of a region harboring an ampC gene encoding a β-lactamase blaDHA-1 as the key determinant of heteroresistance. Individual colonies exhibited variations in the copy number of the genes resulting in resistance level variation which correlated with growth onset (lag times) and growth rates in the presence of CAZ, analysed in linear models. GDA copy number heterogeneity occurred within single resistant colonies, demonstrating heterogeneity of GDA on the single-cell level. The interdependence between GDA, lag time and antibiotic treatment and the strong plasticity underlying heteroresistance underlines the high risk for misdetection of antimicrobial heteroresistance and subsequent treatment failure.
HostingRepository	PRIDE
AnnounceDate	2024-10-17
AnnouncementXML	Submission_2024-10-17_01:07:15.317.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Benno Kuropka
SpeciesList	scientific name: Enterobacter cloacae; NCBI TaxID: 550;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-11 06:32:57	ID requested
⏵ 1	2024-10-17 01:07:15	announced

Dataset with its publication pending

submitter keyword: LC-MS/MS

Marcus Fulde
contact affiliation	Department of Veterinary Medicine, Institute of Microbiology and Epizootics, Centre for Infection Medicine, Freie Universität Berlin, Berlin, Germany
contact email	marcus.fulde@fu-berlin.de
lab head
Benno Kuropka
contact affiliation	Freie Universität Berlin, Institute of Chemistry and Biochemistry, Laboratory of Protein Biochemistry, Thielallee 63, D-14195, Berlin, Germany
contact email	kuropka@zedat.fu-berlin.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD056728
     1. Label: PRIDE project
     2. Name: Heteroresistance in Enterobacter cloacae complex caused by cell-to-cell variation in transient gene duplication-amplification events