PXD056723

PXD056723 is an original dataset announced via ProteomeXchange.

Title	Removal of NHS-labelling by-products in proteomic samples
Description	N-Hydroxysuccinimide chemistry is used extensively across proteomics sample preparation. One of the most popular applications is its use in isobaric reagent based quantitation; specifically both the iTRAQ and TMT approaches which rely on complete labelling of primary amines. However, serines, tyrosines and threonines can also react during TMT labelling procedures. These products are typically classed as over-labelled and are disregarded for quantification. In this study we developed a robust method to remove such over-labelled peptides to improve identification rates and quantitation precision and tested the new method or compatibility with phoshpo-peptides.
HostingRepository	PRIDE
AnnounceDate	2025-03-14
AnnouncementXML	Submission_2025-03-14_06:41:37.779.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yana Demyanenko
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; dehydrated residue; phosphorylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Ascend

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-11 04:10:39	ID requested
⏵ 1	2025-03-14 06:41:38	announced

10.1016/J.MCPRO.2025.100948;

submitter keyword: Phospho-peptides, TMT, N-Hydroxy succinimide, Methylamine,NHS-ester, Hydroxylamine

Shabaz Mohammed
contact affiliation	Rosalind Franklin Institute
contact email	shabaz.mohammed@rfi.ac.uk
lab head
Yana Demyanenko
contact affiliation	Rosalind Franklin Institute
contact email	yana.demyanenko@rfi.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/03/PXD056723

PRIDE project URI

• PRIDE
  1. PXD056723
     1. Label: PRIDE project
     2. Name: Removal of NHS-labelling by-products in proteomic samples