PXD056721

PXD056721 is an original dataset announced via ProteomeXchange.

Title	Hormonal contraceptives and depression: A proteomic analysis using neuronal models
Description	Purpose: Hormonal contraceptives are linked to a higher prevalence of depressive symptoms. Given their popularity in Western countries, understanding the biochemical effects on neuronal cells is crucial to minimizing mental health risks. Experimental design: Neural progenitor cells were treated with ethinyl estradiol (EE) and levonorgestrel (LNG), two synthetic sex hormones commonly used in oral contraception, and S-23, a selective androgen receptor modulator developed as a potential synthetic sex hormone for male hormonal contraception. Label-based quantitative proteomics with the TMTpro 16plex tandem mass tags was used to assess protein expression changes between treated and untreated cells. Results: Treatment of human neural progenitor cells with EE, LNG, EE + LNG, and S 23 led to distinct and overlapping proteomic changes, with enrichment in pathways related to inflammation, oxidative stress, transcriptional regulation, and cell death. Disease association analyses linked these changes to neurodegenerative and psychiatric conditions, including mechanisms relevant to depression. Conclusions and Clinical Relevance: These findings suggest that hormonal compounds used in contraception and performance enhancement may influence molecular pathways implicated in mental health, particularly depression. While not directly translatable to clinical outcomes, the results support the need for further investigation into the neuropsychiatric effects of hormonal treatments.
HostingRepository	PRIDE
AnnounceDate	2026-01-05
AnnouncementXML	Submission_2026-01-04_18:47:08.397.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sam Thilmany
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-11 03:13:33	ID requested
⏵ 1	2026-01-04 18:47:09	announced

Thilmany S, Thomas A, Reinders Y, Shakeri F, Vogel M, Sickmann A, Scholl C, Thevis M, Hormonal Contraceptives and Depression: A Proteomic Analysis Using Neuronal Models. Proteomics Clin Appl, 20(1):e70017(2026) [pubmed]

10.1002/prca.70017;

submitter keyword: oral contraception,major depressive disorder, quantitative proteomics, hormonal contraception, neuronal cell culture

Mario Thevis
contact affiliation	Institute of Biochemistry, Center for Preventive Doping Research, German Sport University Cologne, Cologne, Germany European Monitoring Center for Emerging Doping Agents (EuMoCEDA), Cologne/Bonn, Germany
contact email	thevis@dshs-koeln.de
lab head
Sam Thilmany
contact affiliation	Institute of Biochemistry, German Sport University Cologne, Cologne, Germany Federal Institute for Drugs and Medical Devices, Bonn, Germany
contact email	s.thilmany@biochem.dshs-koeln.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD056721
     1. Label: PRIDE project
     2. Name: Hormonal contraceptives and depression: A proteomic analysis using neuronal models