PXD056720 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | SNX10 regulates the clearance of mitochondrial proteins and mitochondrial bioenergetics |
Description | We here show that SNX10 localizes to endocytic compartments in a PtdIns3P-dependent manner and that mutations in the PX domain associated with autosomal recessive osteopetrosis prevent its endosomal recruitment. We demonstrate that SNX10 regulates endosomal trafficking but also interacts with mitochondrial proteins and shows dynamic interactions with mitochondria. Intriguingly, SNX10 and RAB5A-positive vesicles contain mitochondrial material and stain positive for LC3B. SNX10-positive vesicles contain COX-IV and SAMM50, both proteins being important for mitochondrial respiratory chain function, while other mitochondrial proteins are excluded. We find that depletion of SNX10 results in lower levels of COX-IV and SAMM50 both in vitro and in a zebrafish model, as well as impaired mitochondrial respiration and reduced citrate synthase activity, indicating a role for SNX10 as a regulator of mitochondrial bioenergetics. Importantly, the knockout of SNX10 homologs in zebrafish led to elevated ROS levels and cell death, demonstrating the in vivo relevance of SNX10-mediated regulation of mitochondrial homeostasis. |
HostingRepository | PRIDE |
AnnounceDate | 2025-05-07 |
AnnouncementXML | Submission_2025-05-07_03:35:09.903.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Sachin Singh |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-10-11 02:22:28 | ID requested | |
⏵ 1 | 2025-05-07 03:35:10 | announced | |
Publication List
10.1083/jcb.202404009; |
Trachsel-Moncho L, Veroni C, Mathai BJ, Lapao A, Singh S, Asp NT, Schultz SW, Pankiv S, Simonsen A, SNX10 functions as a modulator of piecemeal mitophagy and mitochondrial bioenergetics. J Cell Biol, 224(5):(2025) [pubmed] |
Keyword List
submitter keyword: mitochondrial homeostasis, autophagy,mitophagy, mitochondria, endosomes, SNX10, endocytosis |
Contact List
Tuula Anneli Nyman |
contact affiliation | Head of the Proteomics Core Facility, NAPI project manager, Institute of Immunology Rikshospitalet, Oslo, Norway |
contact email | tuula.nyman@medisin.uio.no |
lab head | |
Sachin Singh |
contact affiliation | Proteomics core facility, Olso University hospital, Norway |
contact email | sachin.kumar.singh@rr-research.no |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD056720
- Label: PRIDE project
- Name: SNX10 regulates the clearance of mitochondrial proteins and mitochondrial bioenergetics