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PXD056720

PXD056720 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSNX10 regulates the clearance of mitochondrial proteins and mitochondrial bioenergetics
DescriptionWe here show that SNX10 localizes to endocytic compartments in a PtdIns3P-dependent manner and that mutations in the PX domain associated with autosomal recessive osteopetrosis prevent its endosomal recruitment. We demonstrate that SNX10 regulates endosomal trafficking but also interacts with mitochondrial proteins and shows dynamic interactions with mitochondria. Intriguingly, SNX10 and RAB5A-positive vesicles contain mitochondrial material and stain positive for LC3B. SNX10-positive vesicles contain COX-IV and SAMM50, both proteins being important for mitochondrial respiratory chain function, while other mitochondrial proteins are excluded. We find that depletion of SNX10 results in lower levels of COX-IV and SAMM50 both in vitro and in a zebrafish model, as well as impaired mitochondrial respiration and reduced citrate synthase activity, indicating a role for SNX10 as a regulator of mitochondrial bioenergetics. Importantly, the knockout of SNX10 homologs in zebrafish led to elevated ROS levels and cell death, demonstrating the in vivo relevance of SNX10-mediated regulation of mitochondrial homeostasis.
HostingRepositoryPRIDE
AnnounceDate2025-05-07
AnnouncementXMLSubmission_2025-05-07_03:35:09.903.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSachin Singh
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-10-11 02:22:28ID requested
12025-05-07 03:35:10announced
Publication List
10.1083/jcb.202404009;
Trachsel-Moncho L, Veroni C, Mathai BJ, Lapao A, Singh S, Asp NT, Schultz SW, Pankiv S, Simonsen A, SNX10 functions as a modulator of piecemeal mitophagy and mitochondrial bioenergetics. J Cell Biol, 224(5):(2025) [pubmed]
Keyword List
submitter keyword: mitochondrial homeostasis, autophagy,mitophagy, mitochondria, endosomes, SNX10, endocytosis
Contact List
Tuula Anneli Nyman
contact affiliationHead of the Proteomics Core Facility, NAPI project manager, Institute of Immunology Rikshospitalet, Oslo, Norway
contact emailtuula.nyman@medisin.uio.no
lab head
Sachin Singh
contact affiliationProteomics core facility, Olso University hospital, Norway
contact emailsachin.kumar.singh@rr-research.no
dataset submitter
Full Dataset Link List
Dataset FTP location
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