PXD056720

PXD056720 is an original dataset announced via ProteomeXchange.

Title	SNX10 regulates the clearance of mitochondrial proteins and mitochondrial bioenergetics
Description	We here show that SNX10 localizes to endocytic compartments in a PtdIns3P-dependent manner and that mutations in the PX domain associated with autosomal recessive osteopetrosis prevent its endosomal recruitment. We demonstrate that SNX10 regulates endosomal trafficking but also interacts with mitochondrial proteins and shows dynamic interactions with mitochondria. Intriguingly, SNX10 and RAB5A-positive vesicles contain mitochondrial material and stain positive for LC3B. SNX10-positive vesicles contain COX-IV and SAMM50, both proteins being important for mitochondrial respiratory chain function, while other mitochondrial proteins are excluded. We find that depletion of SNX10 results in lower levels of COX-IV and SAMM50 both in vitro and in a zebrafish model, as well as impaired mitochondrial respiration and reduced citrate synthase activity, indicating a role for SNX10 as a regulator of mitochondrial bioenergetics. Importantly, the knockout of SNX10 homologs in zebrafish led to elevated ROS levels and cell death, demonstrating the in vivo relevance of SNX10-mediated regulation of mitochondrial homeostasis.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_03:35:09.903.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sachin Singh
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-11 02:22:28	ID requested
⏵ 1	2025-05-07 03:35:10	announced

10.1083/jcb.202404009;

Trachsel-Moncho L, Veroni C, Mathai BJ, Lapao A, Singh S, Asp NT, Schultz SW, Pankiv S, Simonsen A, SNX10 functions as a modulator of piecemeal mitophagy and mitochondrial bioenergetics. J Cell Biol, 224(5):(2025) [pubmed]

submitter keyword: mitochondrial homeostasis, autophagy,mitophagy, mitochondria, endosomes, SNX10, endocytosis

Tuula Anneli Nyman
contact affiliation	Head of the Proteomics Core Facility, NAPI project manager, Institute of Immunology Rikshospitalet, Oslo, Norway
contact email	tuula.nyman@medisin.uio.no
lab head
Sachin Singh
contact affiliation	Proteomics core facility, Olso University hospital, Norway
contact email	sachin.kumar.singh@rr-research.no
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD056720

PRIDE project URI

• PRIDE
  1. PXD056720
     1. Label: PRIDE project
     2. Name: SNX10 regulates the clearance of mitochondrial proteins and mitochondrial bioenergetics