PXD056630

PXD056630 is an original dataset announced via ProteomeXchange.

Title	Integrated proteomics and lipidomics analysis of hippocampus to reveal the metabolic landscape of epilepsy
Description	Epilepsy is a group of chronic brain disorders characterized by recurrent, abnormal neuronal firing that causes temporary CNS dysfunction. Hippocampal sclerosis (HS) is identified as the predominant pathological alteration in epilepsy, particularly in temporal lobe epilepsy. This study investigates the metabolic profiles of epileptic hippocampal tissues using proteomics and lipidomics techniques. An epilepsy model was established in SD rats via intraperitoneal injection of PTZ, with hippocampal tissue samples subsequently extracted for histopathological examination. Proteomics analysis was conducted using iTRAQ combined with LC-MS/MS, while lipidomics analysis employed UHPLC Q-TOF/MS. Proteomic analysis revealed 144 differentially expressed proteins in acute epileptic hippocampus and 83 in chronic epileptic hippocampus. Key proteins, including Nefh, Vim, Gsn, Sirt2, Cnp, Mef2d, and Ctsd, were pivotal in epileptic hippocampal tissue injury and validated through PRM. Concurrently, lipid metabolomics revealed 83 differentially expressed metabolites in acute epileptic hippocampus and 142 in chronic epileptic hippocampus. Bioinformatics analysis indicated that glycerophospholipid, sphingolipid, and glyceride metabolism pathways were crucial in epileptic hippocampal tissue injury. Integrated proteomics and lipidomics analysis revealed significant correlations between Gsn, Ctsd, and Nefh with four metabolic pathways. These findings provide deeper insights into the pathophysiological mechanisms of epileptic hippocampal tissue damage, potentially unveiling novel therapeutic avenues for clinicians.
HostingRepository	iProX
AnnounceDate	2024-10-09
AnnouncementXML	Submission_2024-10-08_20:28:56.458.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Peng Zhang
SpeciesList	scientific name: Rattus norvegicus; NCBI TaxID: 10116;
ModificationList	iTRAQ8plex reporter+balance reagent acylated residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-08 20:26:43	ID requested
⏵ 1	2024-10-08 20:28:56	announced

Dataset with its publication pending

submitter keyword: hippocampal tissue, epilepsy, proteomics, lipidomics, rats

Peng Zhang
contact affiliation	Hainan Medical University
contact email	972421821@qq.com
lab head
Peng Zhang
contact affiliation	Hainan Medical University
contact email	972421821@qq.com
dataset submitter

iProX dataset URI