PXD056624

PXD056624 is an original dataset announced via ProteomeXchange.

Title	A proteomic map of B cell activation and its shaping by mTORC1, MYC and Iron - Full stimulation, LPS stimulation and rapamycin data
Description	Using high resolution quantitative mass spectrometry we have explored how immune activation and the metabolic checkpoint kinase mTORC1 (mammalian target of rapamycin complex 1) regulate the proteome of B lymphocytes. Triggering the B cell receptor, CD40 and the IL-4 receptor induced considerable re-modelling of the B cell protein landscape, with a 5-fold increase in total cellular protein mass within 24 hours of activation. Analysis of copy numbers per cell of >7,500 proteins revealed the metabolic machinery that supports B cell activation and identified the nutrient and amino acid transporters that fuel B cell biosynthetic capacity. We reveal that mTORC1 controls activation-induced cell growth and B cell proteome remodelling. We show that mTORC1 is critical for the expression of transcription factors that regulate B cell differentiation and metabolism including MYC. mTORC1 activity is required for B cells to sustain MYC expression during activation and inhibiting mTORC1 impairs the expression of nutrient and amino acid transporters that fuel B cell protein production. This work provides a detailed map of naïve and immune activated B cell proteomes and a greater understanding of the cellular machinery that direct B cell phenotypes. This work also provides new insights into the role of mTORC1 and MYC in regulating activation induced proteome remodelling and protein production in B cells.
HostingRepository	PRIDE
AnnounceDate	2026-09-09
AnnouncementXML	Submission_2026-09-09_04:15:40.359.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andrew Howden
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-08 10:28:20	ID requested
⏵ 1	2026-09-09 04:15:40	announced

Dataset with its publication pending

submitter keyword: None

Dr Andy
contact affiliation	School of Life Sciences, University of Dundee, Dundee, UK
contact email	a.howden@dundee.ac.uk
lab head
Andrew Howden
contact affiliation	University of Dundee
contact email	a.howden@dundee.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD056624
     1. Label: PRIDE project
     2. Name: A proteomic map of B cell activation and its shaping by mTORC1, MYC and Iron - Full stimulation, LPS stimulation and rapamycin data