PXD056612
PXD056612 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | AMBRA1 controls the translation of immune-specific genes in T lymphocytes |
| Description | T cell receptor (TCR) engagement causes a global cellular response that entrains signaling pathways, cell cycle regulation, and cell death. The molecular regulation of mRNA translation in these processes is poorly understood. During a whole-genome CRISPR screen for regulators of CD95 (Fas/APO-1)-mediated T cell death, we identified AMBRA1, a protein previously studied for its roles in autophagy, E3 ubiquitin ligase activity, and cyclin regulation. T cells lacking AMBRA1 resist Fas-mediated cell death by down-regulating FAS expression at the translational level. We show that AMBRA1 is a vital regulator of ribosome protein biosynthesis and loading on select mRNAs that plays a key role in balancing TCR signaling with cell cycle regulation pathways. We also found that AMBRA1 itself is translational controlled by TCR stimulation via the CD28-PI3K-mTORC1-EIF4F pathway. Together, these findings shed light on the molecular control of translation after T cell activation and implicate AMBRA1 as a translational regulator that governs TCR signaling, cell cycle progression and T cell death. |
| HostingRepository | MassIVE |
| AnnounceDate | 2024-10-08 |
| AnnouncementXML | Submission_2024-10-08_07:15:29.418.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Bj�rn H�upl |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | Carbamidomethyl; monohydroxylated residue; Acetyl |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-10-08 05:51:45 | ID requested | |
| ⏵ 1 | 2024-10-08 07:15:29 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Protein translation, Fas signaling pathway, AMBRA1, T cell death, T cell activation |
Contact List
| Michael J. Lenardo | |
|---|---|
| contact affiliation | 1Molecular Development of the Immune System Section, Laboratory of Immune System Biology, and Clinical Genomics Program, NIAID, National Institutes of Health, Bethesda, MD 20814 |
| contact email | lenardo@nih.gov |
| lab head | |
| Bj�rn H�upl | |
| contact affiliation | DKFZ/DKTK |
| contact email | b.haeupl@dkfz-heidelberg.de |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v08/MSV000096042/ |
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