PXD056595

PXD056595 is an original dataset announced via ProteomeXchange.

Title	The critical role of MLKL in hemorrhagic stroke and the therapeutic potential of its associated protein network
Description	MLKL, as the executor of necroptosis and a critical factor in the inflammation, has been shown to be associated with the progression of hemorrhagic stroke. Studies identified MLKL is a promoting factor in this process, suggesting its potential as a therapeutic target to mitigate post-hemorrhagic stroke damage. However, there is no direct evidence supporting MLKL as a drug target for hemorrhagic stroke currently, and the precise mechanisms by which MLKL functions in this context remain unclear. Here, we established an intracerebral hemorrhage model by collagenase IV injection and found that MLKL knockout alone was insufficient to fully reverse neuroinflammation and pathological damage. To further elucidate the molecular mechanisms underlying the effects of MLKL knockout on the ICH model, we conducted a proteomic analysis via LC-MS/MS. While MLKL knockout did not significantly reverse the damage caused by intracerebral hemorrhage, enhanced pathways related to inflammation, metabolism, and coagulation as well as attenuated functions related to neuron assemble were identified, suggesting that MLKL may exert its effects through these pathways. Based on these findings, we hypothesize that co-targeting MLKL and its associated protein network may yield better therapeutic outcomes for hemorrhagic stroke.
HostingRepository	iProX
AnnounceDate	2024-09-27
AnnouncementXML	Submission_2024-10-08_01:08:45.130.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jingbo Qie
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-08 01:08:33	ID requested
⏵ 1	2024-10-08 01:08:45	announced

Dataset with its publication pending

submitter keyword: MLKL, hemorrhagic stroke

Jingbo Qie
contact affiliation	Fudan University
contact email	jingboqie@fudan.edu.cn
lab head
Jingbo Qie
contact affiliation	Fudan University
contact email	qiejingbo@126.com
dataset submitter

iProX dataset URI