PXD056594

PXD056594 is an original dataset announced via ProteomeXchange.

Title	Megavirus Translation Initiation Factor 4A Plays an Important Role in the Regulation of Viral and Host Protein Translation and Host Adaptation
Description	The presence of translation-related proteins has long been seen as a critical distinction between viruses and cellular life, the discovery of giant viruses has challenged this standard. We prove that the potential eukaryotic translation initiation factor 4A (eIF4A, Mb0671) encoded by the Megavirus baoshan (M. baoshan), a member of the Mimiviridae family, possesses ATPase activity and RNA-binding capacity. Mb0671 is important for viral adaptation to the host, localizes to the cytoplasm, and is found in mature virions. Proteomics reveals that Mb0671 can manipulate host protein synthesis, compared to wild-type cells, the cellular proteins that are significantly upregulated in A. castellaniia cells overexpressing Mb0671 are significantly enriched in the spliceosome, biosynthesis of amino acids, ribosome biogenesis in eukaryotes, SNARE interactions in vesicular transport, DNA replication, autophagy and mTOR signaling pathway. Proteomics also revealed Mb0671 plays an important role in RNA processing and transport, gene expression regulation in host cells. Proteomics reveals that overexpression of Mb0671 significantly affects the synthesis of viral proteins. Indirect interactions between Mb0671 and the host's eIF4A, along with other interactions primarily with ribosomal and translation-related proteins, suggest that Mb0671 may form a complex with host translation proteins to perform translation functions. Our findings indicate that the virus-encoded translation factor Mb0671 plays an important role in viral protein synthesis, suggesting that these unusual viruses may possess a special translation mechanism in which Mb0671 could play a key role.
HostingRepository	iProX
AnnounceDate	2024-10-03
AnnouncementXML	Submission_2024-10-08_01:05:51.248.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wenya Bian
SpeciesList	scientific name: Mimivirus sp. SH; NCBI TaxID: 2496520;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480; Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-08 01:05:19	ID requested
⏵ 1	2024-10-08 01:05:51	announced

Dataset with its publication pending

submitter keyword: Megavirus, Putative Translation Initiation Factor 4A, Proteomics

Jiang Zhong
contact affiliation	Fudan University
contact email	jzhong@fudan.edu.cn
lab head
Wenya Bian
contact affiliation	Fudan University
contact email	21110700002@m.fudan.edu.cn
dataset submitter

iProX dataset URI