PXD056592

PXD056592 is an original dataset announced via ProteomeXchange.

Title	Identification of SpySystem-linked CYP73A5-ATR2 by LC-MSMS
Description	Eukaryotic cytochrome P450 enzymes, generally colocalizing with their redox partner cytochrome P450 reductase (CPR) on the cytoplasmic surface of organelle membranes, often perform poorly in prokaryotic cells, whether expressed with CPR as a tandem chimera or free-floating individuals, causing a low titer of heterologous chemicals. To improve their biosynthetic performance in Escherichia coli, we here architecturally design self-assembled alternatives of eukaryotic P450 system using reconstructed P450 and CPR, and create a set of N-termini-bridged P450-CPR heterodimers as the counterparts of eukaryotic P450 system with N-terminus-guided colocalization. The covalent counterparts show superior and robust biosynthetic performance; the N-termini-bridged architecture is validated to improve the biosynthesis of both plant and human P450 systems. Furthermore, the architectural configuration of protein assemblies has an inherent effect on the biosynthesis of N-termini-bridged P450-CPR heterodimers. The results suggest that spatial architecture-guided protein assembly could serve as an efficient strategy for improving the biosynthesis of protein complexes, particularly those related to eukaryotic membranes, in prokaryotic and even eukaryotic hosts.
HostingRepository	iProX
AnnounceDate	2024-10-04
AnnouncementXML	Submission_2024-10-08_01:02:28.464.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yikui Li
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-08 01:01:56	ID requested
⏵ 1	2024-10-08 01:02:28	announced

Dataset with its publication pending

submitter keyword: Eukaryotic P450 system, Spatial organization, Protein architecture, Electron transfer, Biosynthesis, Escherichia coli

Ren Wang
contact affiliation	Institute of Botany, Jiangsu Province and Chinese Academy of Sciences
contact email	rwang@cnbg.net
lab head
Yikui Li
contact affiliation	Institute of Botany, Jiangsu Province and Chinese Academy of Sciences
contact email	liyikui@cnbg.net
dataset submitter

iProX dataset URI