PXD056588

PXD056588 is an original dataset announced via ProteomeXchange.

Title	Identification of TRADD phosphorylation sites
Description	We re-introduced Flag-mTRADD into Tradd-/- MEFs, and identified the phosphorylation sites of TRADD which were induced by TNF and inhibited by TAK1 inhibitor 5Z7 using mass spectrometry. We found that TAK1 could mediate the phosphorylation of multiple sites in TRADD upon TNF stimulation. We next performed in vitro kinase assay using recombinant human TAK1 and human TRADD, followed by mass spectrometry to identify the sites directly phosphorylated by TAK1.
HostingRepository	iProX
AnnounceDate	2024-10-05
AnnouncementXML	Submission_2025-09-03_00:18:42.746.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bing Shan
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-08 00:56:47	ID requested
1	2024-10-08 00:56:58	announced
⏵ 2	2025-09-03 00:18:43	announced	2025-09-03: Add info.

Sun Z, Ye J, Sun W, Jiang L, Shan B, Zhang M, Xu J, Li W, Liu J, Jing H, Zhang T, Hou M, Xie C, Wu R, Pan H, Yuan J, Cooperation of TRADD- and RIPK1-dependent cell death pathways in maintaining intestinal homeostasis. Nat Commun, 16(1):1890(2025) [pubmed]

submitter keyword: TRADD binding phosphorylation sites

Junying Yuan
contact affiliation	Shanghai Institute of Organic Chemistry, CAS
contact email	junying_yuan@sioc.ac.cn
lab head
Bing Shan
contact affiliation	Shanghai Institute of Organic Chemistry, CAS
contact email	shanbing@sioc.ac.cn
dataset submitter

iProX dataset URI