PXD056558

PXD056558 is an original dataset announced via ProteomeXchange.

Title	Proteomic profiling of tissue and plasma from mares with endometrial fibrosis
Description	Endometrial fibrosis causes subfertility in mares, and is characterized by excessive deposition of extracellular matrix. It is diagnosed by a histopathologic evaluation of a biopsy, but the prognostic value of a single biopsy is limited, and a better understanding of the pathogenesis is needed. To gain a better understanding of protein dynamics underlying this disease, Data-Independent Acquisition (DIA) Mass Spectrometry was utilized to comprehensively character-ize and compare the equine endometrial and plasma proteome from these mares. Mares in die-strus were selected as controls (n=9), with moderate endometrial fibrosis (n=9), and severe endometrial fibrosis (n=9), based on tissue sections stained with hematoxylin and eosin and immunostained for alpha-smooth muscle actin. Approximately 10,000 proteins were identified in endometrial tissue and 500 in plasma, from which 310 and 75 were significantly regulated between moderate endometrial fibrosis and controls, and 585 and 71 between severe endome-trial fibrosis and controls. Pathways involved in extracellular matrix were over-represented among upregulated proteins in endometrial tissue and among downregulated proteins in plasma from moderate and severe endometrial fibrosis compared to controls. Several of the proteins involved appeared to be driving the development or progression of endometrial fibrosis. Path-ways involved in the immune response were over-represented among downregulated proteins in moderate endometrial fibrosis, and among upregulated proteins in severe endometrial fibro-sis, in both endometrial tissue and plasma. Several of the proteins highlighted by our analysis appear to have both immunologic and fibrotic properties. This extensive profiling of the endo-metrial and plasma proteome paves the way for further research in potential biomarkers and candidates for therapeutic use in endometrial fibrosis.
HostingRepository	PRIDE
AnnounceDate	2026-01-20
AnnouncementXML	Submission_2026-01-19_21:56:41.336.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rune Daucke
SpeciesList	scientific name: Equus caballus (Horse); NCBI TaxID: NEWT:9796;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-07 10:36:11	ID requested
⏵ 1	2026-01-19 21:56:41	announced

10.1093/BIOLRE/IOAF217;

submitter keyword: endometrial proteome, fibrotic endometrial degeneration, data independent acquisition mass spectrometry,equine, reproduction

Erwin Marten Schoof
contact affiliation	Technical University of Denmark, Department of Biotechnology and Biomedicine, Section for Medical Biotechnology, Cell diversity Lab
contact email	erws@dtu.dk
lab head
Rune Daucke
contact affiliation	Technical University of Denmark
contact email	runda@dtu.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD056558

PRIDE project URI

• PRIDE
  1. PXD056558
     1. Label: PRIDE project
     2. Name: Proteomic profiling of tissue and plasma from mares with endometrial fibrosis