PXD056546

PXD056546 is an original dataset announced via ProteomeXchange.

Title	Proteomic Profiling of Dysbiosis-Challenged Broilers: Blood Biomarkers for Intestinal Health
Description	The intestinal microbiome forms dynamic ecosystem whose balanced composition and functioning are essential for maintaining overall gut health and well-being in living organisms. In broilers, dysbiosis disrupts the microbiota-host balance, often without obvious clinical symptoms but with intestinal inflammation, leading to impaired animal performance and significant economic losses. This study utilizes an in vivo model of dysbiosis to investigate the blood proteome response of broilers to intestinal imbalance. Microscopic histological changes in the gut (shorter villi, increased crypt depth, p<0.0001) were observed in the duodenal and jejunal tissue of challenged birds. Elevated levels of permeability markers faecal ovotransferrin (p < 0.0001) and serum iohexol (p= 0.0009) additionally indicated increased intestinal permeability in challenged group compared to control. The MS/MS-based proteomics analysis was performed on broilers’ blood plasma enabling identification of 388 proteins, 25 of which demonstrated significant difference between the groups. Functional analysis showed activation of immune response, signalling, and interspecies interaction, while proteins related to cellular physiology, cell-cell communication, and extracellular matrix (ECM) processes were suppressed. Protein-protein interaction (PPI) analysis revealed two clusters of downregulated proteins involved in ECM organization and cell adhesion. These results suggest that the dysbiosis challenge alters plasma protein expression as the host prioritizes immune defense over structural maintenance. The activation of immune processes and suppression of ECM pathways highlight potential biomarkers and therapeutic targets for addressing dysbiosis.
HostingRepository	PRIDE
AnnounceDate	2025-07-14
AnnouncementXML	Submission_2025-07-13_16:36:05.347.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Teresa Mendes Maia
SpeciesList	scientific name: Gallus gallus (Chicken); NCBI TaxID: 9031;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-07 06:57:01	ID requested
⏵ 1	2025-07-13 16:36:06	announced

10.1186/s13567-025-01570-4;

Tretiak S, Mendes Maia T, Ducatelle R, Cherlet M, Rijsselaere T, Van Immerseel F, Impens F, Antonissen G, Proteomic profiling of dysbiosis-challenged broilers reveals potential blood biomarkers for intestinal health. Vet Res, 56(1):143(2025) [pubmed]

submitter keyword: intestinal permeability, broiler chicken, proteomics, dysbiosis, gut health,Blood biomarkers

Francis Impens
contact affiliation	VIB-UGent Center for Medical Biotechnology UGent Department of Biomolecular Medicine Technologiepark-Zwijnaarde 75 B9052 Gent Belgium
contact email	Francis.Impens@vib-ugent.be
lab head
Teresa Mendes Maia
contact affiliation	VIB Proteomics Core
contact email	teresa.maia@vib-ugent.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD056546

PRIDE project URI

• PRIDE
  1. PXD056546
     1. Label: PRIDE project
     2. Name: Proteomic Profiling of Dysbiosis-Challenged Broilers: Blood Biomarkers for Intestinal Health