PXD056526

PXD056526 is an original dataset announced via ProteomeXchange.

Title	Chemoproteomic identification of a phosphohistidine acceptor: Insights into posttranslational regulation of glycolysis
Description	Histidine phosphorylation is an underexplored form of protein phosphorylation. Despite the widespread existence of phosphohistidine (pHis) sites, particularly in bacteria, their phosphorylation regulation and physiological functions remain poorly understood. In this study, we developed a chemoproteomic strategy employing a stable pHis analog to identify pHis-recognizing proteins in Escherichia coli. Our probe successfully labeled known pHis-recognizers and revealed many putative pHis acceptors, including phosphofructokinase-1 (PfkA), a key glycolytic enzyme. We demonstrated that PfkA undergoes histidine phosphorylation at His249 mediated by the phosphocarrier protein PtsH, thereby reducing enzyme activity. This phosphorylation was reversed by the pHis-specific phosphatase SixA, which restored the PfkA activity. Our findings reveal a novel posttranslational regulatory mechanism affecting glycolysis, implicating a broader role of histidine phosphorylation in bacterial metabolic control.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_03:32:20.320.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Seungmin Ahn
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-06 04:57:17	ID requested
⏵ 1	2025-05-07 03:32:21	announced

10.1039/d5sc01024a;

Choi S, Ahn S, Cho KH, Lee SK, Kee JM, Chemoproteomic identification of phosphohistidine acceptors: posttranslational activity regulation of a key glycolytic enzyme. Chem Sci, 16(18):8014-8022(2025) [pubmed]

submitter keyword: Cell lysate,E.coli, Qexactive, LC-MS/MS

Jung-Min Kee
contact affiliation	Associate professor, Department of Chemistry, Ulsan National Institute of Science and Technology (UNIST)
contact email	jmkee@unist.ac.kr
lab head
Seungmin Ahn
contact affiliation	Ulsan National Institute of Science and Technology (UNIST)
contact email	asm8430@naver.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD056526
     1. Label: PRIDE project
     2. Name: Chemoproteomic identification of a phosphohistidine acceptor: Insights into posttranslational regulation of glycolysis