PXD056491

PXD056491 is an original dataset announced via ProteomeXchange.

Title	USP37 protects mammalian cells during DNA replication stress by counteracting CUL2-LRR1 and TRAIP
Description	We have found that the USP37 deubiquitylase binds to the DNA helicase known as CMG (Cdc45-Mcm-GINS) in mammalian cells. CMG unwinds the parental DNA duplex during the process of chromosome duplication, and USP37 is important to help cells survive problems in DNA replication. In this project, we investigated the partners of the mammalian CMG helicase. We compared control mouse embryonic stem cells (mES cells) to mES cells in which the SLD5 component of the CMG helicase was fused to the ‘Tandem Affinity Purification’ tag (TAP-tag). Cells were either untreated or exposed to 5 µM CB-5083 for three hours, to inhibit activity of the p97 ATPase.
HostingRepository	PRIDE
AnnounceDate	2026-06-09
AnnouncementXML	Submission_2026-06-09_05:08:59.544.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD056491
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	KARIM LABIB
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-03 19:04:21	ID requested
⏵ 1	2026-06-09 05:09:00	announced

10.6019/PXD056491;

10.1016/j.celrep.2025.115739;

Villa F, Ainsworth J, Labib KPM, and TRAIP. Cell Rep, 44(6):115739(2025) [pubmed]

submitter keyword: USP37, ATR checkpoint kinase, Ubiquitylation, Deubiquitylase, TRAIP, CMG helicase, CUL2-LRR1, DNA replication

Professor Karim
contact affiliation	MRC Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences, University of Dundee, DD1 5EH, U.K.
contact email	kpmlabib@dundee.ac.uk
lab head
KARIM LABIB
contact affiliation	University of Dundee
contact email	kpmlabib@dundee.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD056491
     1. Label: PRIDE project
     2. Name: USP37 protects mammalian cells during DNA replication stress by counteracting CUL2-LRR1 and TRAIP