PXD056490

PXD056490 is an original dataset announced via ProteomeXchange.

Title	Serine supplementation suppresses hypoxia-induced pathological retinal angiogenesis
Description	Pathological retinal angiogenesis with irregular and fragile vessels (also termed as neovascularization, a response to hypoxia and dysmetabolism) is a leading cause of vision loss in all age groups driven in part by unmet metabolic demand from retinal neurons. Sustaining neural retinal metabolism with an adequate nutrient supply may prevent vision-threatening neovascularization. Low circulating serine levels are associated with neovascularization in macular telangiectasia and altered serine/glycine metabolism is suggested in retinopathy of prematurity. Here we assessed the role of serine metabolism in suppressing hypoxia-driven retinal neovascularization in mice. Systemic serine supplementation decreased, and dietary serine/glycine deficiency worsened retinal neovascularization. Fatty acid oxidation was essential in mediating serine protective effects and serine also increased the levels of phosphatidylcholine, the most abundant phospholipids in the retina. Exogenous serine increased abundance of proteins involved in oxidative phosphorylation in total retinas, as well as increased expression of mitochondrial respiration-related genes and decreased expression of pro-angiogenic genes in rod photoreceptor cluster. Pharmaceutical inhibition of mitochondrial energy production largely attenuated serine suppression of retinal neovascularization. Our data suggested that increasing serine is a potential therapeutic approach for neovascular eye diseases by enhancing retinal mitochondrial function and lipid metabolism to suppress driving factors for uncontrolled angiogenesis.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_03:46:22.592.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD056490
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Zhongjie Fu
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-03 12:39:03	ID requested
⏵ 1	2025-05-07 03:46:23	announced

10.7150/thno.105299;

Yagi H, Qiu C, Zeng Y, Boeck M, Nian S, Chen CT, Harman JC, Kasai T, Lee J, Hirst V, Neilsen K, Wang C, Bora K, Maurya M, Rodrick T, Grumbine M, Yang Y, Hua Z, Sweet IR, Singh SA, Aikawa M, Chen J, Fu Z, Serine supplementation suppresses hypoxia-induced pathological retinal angiogenesis. Theranostics, 15(11):5087-5105(2025) [pubmed]

10.6019/PXD056490;

submitter keyword: retinopathy of prematurity,serine, neovascularization, angiogenesis, retina

Zhongjie Fu
contact affiliation	Boston Children's Hospital
contact email	Zhongjie.fu@childrens.harvard.edu
lab head
Zhongjie Fu
contact affiliation	Boston Children's Hospital
contact email	zhongjie.fu@childrens.harvard.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD056490
     1. Label: PRIDE project
     2. Name: Serine supplementation suppresses hypoxia-induced pathological retinal angiogenesis