PXD056473 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification by activity-based probes of a new Plasmodium falciparum E2 ubiquitin conjugating enzyme |
Description | The ubiquitin-proteasome system (UPS) is essential for Plasmodium falciparum survival and represents a potential target for antimalarial therapies. We utilized a ubiquitin-based activity probe (Ub-Dha) to capture active ubiquitin conjugating machinery during asexual blood-stage development. Several E2 ubiquitin-conjugating enzymes, the E1 activating enzyme, and the HECT E3 ligase PfHEUL were identified and validated through in vitro ubiquitination assays. We also demonstrate selective functional interactions between PfHEUL and a subset of both human and P. falciparum E2s. Additionally, the Ub-Dha probe captured an uncharacterized protein, C0H4U0_PLAF7 with no known homology to ubiquitin-pathway enzymes in other organisms. Through structural and biochemical analysis, we validate it as a novel E2 enzyme, capable of binding ubiquitin in a cysteine-specific manner. These findings contribute to our understanding of the P. falciparum UPS, identifying promising novel drug targets and highlighting the evolutionary uniqueness of the Ub-proteasome system in this parasite. |
HostingRepository | PRIDE |
AnnounceDate | 2025-03-13 |
AnnouncementXML | Submission_2025-03-13_05:52:26.704.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Adan Pinto-Fernandez |
SpeciesList | scientific name: Plasmodium falciparum (isolate 3D7); NCBI TaxID: 36329; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-10-03 04:29:18 | ID requested | |
⏵ 1 | 2025-03-13 05:52:27 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Plasmodium Falciparum, Activity Proteomics, Ubiquitin |
Contact List
Adan Pinto-Fernandez |
contact affiliation | COI-NDM Univesity of Oxford |
contact email | adan.pintofernandez@ndm.ox.ac.uk |
lab head | |
Adan Pinto-Fernandez |
contact affiliation | University of Oxford |
contact email | adan.pintofernandez@ndm.ox.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/03/PXD056473 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD056473
- Label: PRIDE project
- Name: Identification by activity-based probes of a new Plasmodium falciparum E2 ubiquitin conjugating enzyme