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PXD056473

PXD056473 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification by activity-based probes of a new Plasmodium falciparum E2 ubiquitin conjugating enzyme
DescriptionThe ubiquitin-proteasome system (UPS) is essential for Plasmodium falciparum survival and represents a potential target for antimalarial therapies. We utilized a ubiquitin-based activity probe (Ub-Dha) to capture active ubiquitin conjugating machinery during asexual blood-stage development. Several E2 ubiquitin-conjugating enzymes, the E1 activating enzyme, and the HECT E3 ligase PfHEUL were identified and validated through in vitro ubiquitination assays. We also demonstrate selective functional interactions between PfHEUL and a subset of both human and P. falciparum E2s. Additionally, the Ub-Dha probe captured an uncharacterized protein, C0H4U0_PLAF7 with no known homology to ubiquitin-pathway enzymes in other organisms. Through structural and biochemical analysis, we validate it as a novel E2 enzyme, capable of binding ubiquitin in a cysteine-specific manner. These findings contribute to our understanding of the P. falciparum UPS, identifying promising novel drug targets and highlighting the evolutionary uniqueness of the Ub-proteasome system in this parasite.
HostingRepositoryPRIDE
AnnounceDate2025-03-13
AnnouncementXMLSubmission_2025-03-13_05:52:26.704.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAdan Pinto-Fernandez
SpeciesList scientific name: Plasmodium falciparum (isolate 3D7); NCBI TaxID: 36329;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-10-03 04:29:18ID requested
12025-03-13 05:52:27announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Plasmodium Falciparum, Activity Proteomics, Ubiquitin
Contact List
Adan Pinto-Fernandez
contact affiliationCOI-NDM Univesity of Oxford
contact emailadan.pintofernandez@ndm.ox.ac.uk
lab head
Adan Pinto-Fernandez
contact affiliationUniversity of Oxford
contact emailadan.pintofernandez@ndm.ox.ac.uk
dataset submitter
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Dataset FTP location
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