PXD056466 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | SMYD5 is a ribosomal methyltransferase which trimethylates RPL40 lysine 22 through recognition of a KXY motif |
Description | The eukaryotic ribosome is highly modified by protein methylation, yet many of the responsible methyltransferases remain unknown. Here we have identified SMYD5 as a ribosomal protein methyltransferase that catalyses trimethylation of RPL40 at lysine 22. Through a systematic mass spectrometry-based approach, we show that the human ribosome has 12 primary sites of protein methylation, including at RPL40 K22. Through in vitro methylation of synthetic RPL40 using fractionated lysate, we then identify SMYD5 as a candidate RPL40 K22 methyltransferase. We show that recombinant SMYD5 has robust activity towards RPL40 K22 in vitro, and that active site mutations ablate this activity. Knockouts of SMYD5 in K562 cells show a complete loss of RPL40 K22 methylation and decrease polysome levels. By systematic analysis of its recognition motif, we show that SMYD5 requires a tyrosine in the +2 position, and thereby is incapable of methylating its previously reported histone substrates. |
HostingRepository | PRIDE |
AnnounceDate | 2025-04-07 |
AnnouncementXML | Submission_2025-04-06_21:15:07.371.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Joshua Hamey |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monomethylated residue; N6; dimethylated residue |
Instrument | Orbitrap Fusion Lumos; LTQ Orbitrap Velos; Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-10-02 19:41:11 | ID requested | |
⏵ 1 | 2025-04-06 21:15:07 | announced | |
Publication List
Keyword List
submitter keyword: Ribosome, lysine methylation, substrate specificity, translation, methyltransferase |
Contact List
Marc Wilkins |
contact affiliation | University of New South Wales |
contact email | m.wilkins@unsw.edu.au |
lab head | |
Joshua Hamey |
contact affiliation | University of New South Wales |
contact email | j.hamey@unsw.edu.au |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD056466
- Label: PRIDE project
- Name: SMYD5 is a ribosomal methyltransferase which trimethylates RPL40 lysine 22 through recognition of a KXY motif