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PXD056465

PXD056465 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleEstablishment of Minimum Protein Standards for Mycobacterium tuberculosis-Derived Extracellular Vesicles through Comparison of EV Enrichment Methods
DescriptionExtracellular vesicles (EVs) produced by Mycobacterium tuberculosis, the causative agent of tuberculosis, are involved in acquiring nutrients, evading host immune mechanisms, and modulating the host immune response. The functions of these M. tuberculosis EVs (MEVs)are hypothesized to be due to the dynamic composition of MEVs. However, there are various methods used to purify MEVs, and these methods can impact MEV characteristics such as size, purity, integrity, and composition. In this study, we investigated several methods for MEV purification, including: ultracentrifugation, differential density gradient-based ultracentrifugation, qEV size exclusion chromatography, and Capto(TM)Core size exclusion chromatography. We evaluated the size, concentration, appearance, purity, and protein composition (by mass spectrometry) of MEVs from each of these methods. We ultimately found that protein and particle yield vary across methods and that the size exclusion chromatography-based methods are more technically reproducible than the ultracentrifugation-based methods. Through mass spectrometry analysis, we also identified seven M. tuberculosis proteins that were present regardless of MEV enrichment method, that we propose could be used as qualitative markers of MEVs versus contaminants (in addition to MEV size and appearance criteria), to benefit the reproducibility of ongoing MEV studies.
HostingRepositoryPRIDE
AnnounceDate2025-05-07
AnnouncementXMLSubmission_2025-05-07_03:53:59.714.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterKimberly Shelton
SpeciesList scientific name: Mycobacterium tuberculosis H37Rv; NCBI TaxID: 83332;
ModificationListNo PTMs are included in the dataset
InstrumenttimsTOF Pro
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-10-02 14:37:39ID requested
12025-05-07 03:54:00announced
Publication List
Ryan JM, Shelton K, Dzieciatkowska M, Kruh-Garcia N, Dobos KM, -derived extracellular vesicles through comparison of EV enrichment methods. Mycobacteria, 1(1):3(2025) [pubmed]
10.1186/s44350-025-00003-8;
Keyword List
submitter keyword: proteomics,Mycobacterium tuberculosis, liquid chromatography-mass spectrometry/mass spectrometry, extracellular vesicles, Bruker timsTOF Pro
Contact List
Karen M Dobos
contact affiliationDepartment of Microbiology, Immunology, and Pathology, Mycobacteria Research Laboratories, Colorado State University, Colorado, USA
contact emailkaren.dobos@colostate.edu
lab head
Kimberly Shelton
contact affiliationColorado State University
contact emailkimberly.shelton@colostate.edu
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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