PXD056445

PXD056445 is an original dataset announced via ProteomeXchange.

Title	LAP1 interactome profiling provides new insights into LAP1’s physiological functions
Description	The nuclear envelope (NE), a protective membrane bordering the nucleus, is composed of highly specialized proteins that are indispensable for normal cellular activity. Lamina-associated polypeptide 1 (LAP1) is a NE protein whose functions are just beginning to be unveiled. The fact that mutations causing LAP1 deficiency are extremely rare and pathogenic is indicative of its paramount importance to preserve human health, anticipating that LAP1 might have a multifaceted role in the cell. Mapping the LAP1 protein interactome is, thus, imperative to achieve an integrated view of its potential biological properties. To this end, we employed in silico- and mass spectrometry-based approaches to identify candidate LAP1-interacting proteins, whose functional attributes were subsequently characterized using bioinformatics tools. Our results suggest that LAP1 may participate in cellular processes as diverse as chromatin and cytoskeleton organization, DNA repair, RNA processing, translation as well as protein biogenesis and turnover. Novel interactions between LAP1 and DNA repair proteins were additionally validated, strengthening the previously proposed involvement of LAP1 in the maintenance of genomic stability. Overall, the data presented in this study reaffirm the biological relevance of LAP1 and the need to deepen our knowledge about this NE protein, providing new insights that will help guiding future research towards a mechanistic understanding of LAP1’s functioning.
HostingRepository	PRIDE
AnnounceDate	2025-01-13
AnnouncementXML	Submission_2025-01-13_01:03:36.226.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	guadalupe espadas
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-02 04:49:53	ID requested
⏵ 1	2025-01-13 01:03:36	announced

10.3390/IJMS252413235;

submitter keyword: DNA repair, interactions

Eduard Sabidó
contact affiliation	Centre for Genomic Regulation (CRG)
contact email	eduard.sabido@crg.eu
lab head
guadalupe espadas
contact affiliation	crg
contact email	guadalupe.espadas@crg.eu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD056445
     1. Label: PRIDE project
     2. Name: LAP1 interactome profiling provides new insights into LAP1’s physiological functions