PXD056424

PXD056424 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Effect of capacitation on proteomic profile and mitochondrial parameters of spermatozoa in bulls
Description	Sperm capacitation is a critical process for fertilization. This process involves several biochemical and physiological changes that have been extensively studied, but little is known about the changes that occur in the sperm’s proteomic profile. This work aims to analyze the effect in vitro capacitation had on the proteome and mitochondrial parameters of bull spermatozoa. Samples of non-capacitated (NC) and in-vitro capacitated (ICV) sperm were analyzed using flow cytometry to assess sperm viability, mitochondrial membrane potential (MMP), and reactive oxygen species (mROS). Proteome was evaluated using SWATH-MS. In-vitro capacitation induced a significant decrease in sperm viability, high MMP, and a significant increase in mROS production. Within the group of living spermatozoa, the capacitation process induced a significant decrease in healthy mitochondrial spermatozoa, as well as a significant increase in mROS production, without affecting the MMP intensity
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_03:27:18.432.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Luz Valero
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 6600

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-01 06:01:45	ID requested
⏵ 1	2025-05-07 03:27:19	announced

Publication List

10.1021/acs.jproteome.4c00910;
Castell, ó, -Ruiz M, Gacem S, S, á, nchez Del Pino MM, Hidalgo CO, Tamargo C, Á, lvarez-Rodr, í, guez M, Y, á, niz JL, Silvestre MA, Effect of Capacitation on Proteomic Profile and Mitochondrial Parameters of Spermatozoa in Bulls. J Proteome Res, 24(4):1817-1831(2025) [pubmed]

10.1021/acs.jproteome.4c00910;

Castell, ó, -Ruiz M, Gacem S, S, á, nchez Del Pino MM, Hidalgo CO, Tamargo C, Á, lvarez-Rodr, í, guez M, Y, á, niz JL, Silvestre MA, Effect of Capacitation on Proteomic Profile and Mitochondrial Parameters of Spermatozoa in Bulls. J Proteome Res, 24(4):1817-1831(2025) [pubmed]

Keyword List

submitter keyword: bovine,spermatozoa, mitochondria, proteome

submitter keyword: bovine,spermatozoa, mitochondria, proteome

Contact List

Miguel Ángel Silvestre
contact affiliation	Department of Cell Biology, Functional Biology and Physical Anthropology Universitat de València, 46100 Valencia, Spain.
contact email	miguel.silvestre@uv.es
lab head
Luz Valero
contact affiliation	Universitat de Valencia
contact email	mluz.valero@uv.es
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD056424
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD056424

PRIDE project URI

Repository Record List

[ + ]