PXD056417

PXD056417 is an original dataset announced via ProteomeXchange.

Title	ATG9A TurboID - ATG9A and ARFIP2 cooperate to regulate PI4P levels for lysosomal repair
Description	Lysosome damage activates multiple pathways to prevent lysosome-dependent cell death, including a repair mechanism involving ER-lysosome membrane contact sites, phosphatidylinositol 4-kinase-2a (PI4K2A), phosphatidylinositol-4 phosphate (PI4P) and oxysterol-binding protein-related proteins (ORPs) lipid transfer proteins. PI4K2A localizes to trans-Golgi network and endosomes yet how it is delivered to damaged lysosomes remains unknown. During acute sterile damage, and damage caused by intracellular bacteria, we show that ATG9A-containing vesicles perform a critical role in delivering PI4K2A to damaged lysosomes. ADP ribosylation factor interacting protein 2 (ARFIP2), a component of ATG9A vesicles, binds and sequesters PI4P on lysosomes, balancing ORP-dependent lipid transfer and promoting retrieval of ATG9A vesicles through recruitment of the adaptor protein complex-3 (AP-3). Our results reveal a role for mobilized ATG9A vesicles and ARFIP2 in lysosome homeostasis after damage and bacterial infection.
HostingRepository	PRIDE
AnnounceDate	2025-04-22
AnnouncementXML	Submission_2025-04-22_01:32:38.235.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Eugenia Almacellas
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-01 02:06:56	ID requested
⏵ 1	2025-04-22 01:32:38	announced

Dataset with its publication pending

submitter keyword: ARFIP2, membrane trafficking, autophagy,lysosomal damage, lysosome, AP-3, PI4K2A, ATG9A, PI4P

Sharon A. Tooze
contact affiliation	Molecular Cell Biology of Autophagy Laboratory, The Francis Crick Institute, London, United Kingdom
contact email	sharon.tooze@crick.ac.uk
lab head
Eugenia Almacellas
contact affiliation	The Francis Crick institute
contact email	eugenia.almacellasicanals@crick.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/04/PXD056417

PRIDE project URI

• PRIDE
  1. PXD056417
     1. Label: PRIDE project
     2. Name: ATG9A TurboID - ATG9A and ARFIP2 cooperate to regulate PI4P levels for lysosomal repair