PXD056391

PXD056391 is an original dataset announced via ProteomeXchange.

Title	Identification of potential uranium-binding proteins in Arabidopsis thaliana by a 2-dimensional gel electrophoresis-based approach
Description	Uranium is metal that appears more and more as a soil contaminant, on the one hand because of the increased extraction of uranium worldwide for nuclear energy production, and on the other hand as a contaminant of phosphate fertilizers. Uranium is known to alter plant physiology, but the molecular actors implied in these alterations, i.e. the uranium-binding proteins, are poorly known up to now. In order to improve our knowledge on this topic, a 2D gel-based approach relying on protein correlation profiling was used. To this purpose, Arabidopsis thaliana cells were cultivated under photosynthetic conditions until a sufficient number of cells was reached. The cells were then rinsed and exposed to 50µM uranyl nitrate in a Murashige and Skoog medium deprived of phosphate or containing 30µM phosphate. The cells were then washed with 10mM sodium carbonate, twice with distilled water and kept frozen until use.
HostingRepository	PRIDE
AnnounceDate	2026-02-09
AnnouncementXML	Submission_2026-02-09_01:17:38.261.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD056391
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Hélène Diemer
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: NEWT:3702;
ModificationList	iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-30 06:26:08	ID requested
⏵ 1	2026-02-09 01:17:38	announced

10.1016/J.JHAZMAT.2025.139163;

10.6019/PXD056391;

submitter keyword: Uranium, Arabidospsis thaliana, 2D gel, LC-MS/MS

Sarah CIANFERANI
contact affiliation	Laboratoire de Spectrometrie de Masse BioOrganique (LSMBO), Universite de Strasbourg, CNRS, IPHC UMR 7178, 67087 Strasbourg, France
contact email	sarah.cianferani@unistra.fr
lab head
Hélène Diemer
contact affiliation	LSMBO
contact email	hdiemer@unistra.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD056391

PRIDE project URI

• PRIDE
  1. PXD056391
     1. Label: PRIDE project
     2. Name: Identification of potential uranium-binding proteins in Arabidopsis thaliana by a 2-dimensional gel electrophoresis-based approach