PXD056370

PXD056370 is an original dataset announced via ProteomeXchange.

Title	Teleost spermatozoa can conduct de novo cytoplasmic and mitochondrial translation during post-testicular maturation
Description	Translational silence of spermatozoa has long been considered the norm in animals. However, studies in mammals have shown that the mitochondrial ribosomal machinery is selectively activated during capacitation in the female reproductive tract, while cytoplasmic ribosomes remain inactive. Here, using quantitative proteomics in a piscine model species, we show that proteins involved in mRNA processing and cytoplasmic translation are predominantly accumulated in maturing spermatozoa within the extratesticular excurrent ducts, while those related to flagellar motility are enriched in mature (ejaculated) sperm. Based upon in vitro incubation of spermatozoa, motility assays and polysome profiling, we further show that 80S cytoplasmic and 55S mitochondrial ribosomes are actively involved in the translation of motility- and osmoadaptation-related proteins. These findings thus reveal that piscine spermatozoa can maintain de novo protein synthesis through both mitochondrial and cytoplasmic ribosomal activity during post-testicular maturation, which is necessary for the acquisition of full sperm function.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_03:21:19.268.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Montserrat Carrascal
SpeciesList	scientific name: Sparus aurata; NCBI TaxID: 8175;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion; LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-30 01:30:54	ID requested
⏵ 1	2025-05-07 03:21:20	announced

Castro-Arnau J, Chauvign, é F, Gonz, á, lez A, Finn RN, Carrascal M, Cerd, à J, cytoplasmic and mitochondrial translation. iScience, 28(1):111537(2025) [pubmed]

10.1016/j.isci.2024.111537;

submitter keyword: proteomics, efferent duct, sperm motility, polysomes,Fish, LC-MS/MS

Joan Cerda
contact affiliation	1 Institute of Marine Sciences, Spanish National Research Council (CSIC), 08003 Barcelona, Spain 2 Institute of Biotechnology and Biomedicine (IBB), Universitat Autònoma de Barcelona, 08193 Bellaterra (Cerdanyola del Vallès), Spain
contact email	jcerda@icm.csic.es
lab head
Montserrat Carrascal
contact affiliation	IIBB-CSIC
contact email	montserrat.carrascal.csic@uab.cat
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD056370

PRIDE project URI

• PRIDE
  1. PXD056370
     1. Label: PRIDE project
     2. Name: Teleost spermatozoa can conduct de novo cytoplasmic and mitochondrial translation during post-testicular maturation