PXD056356

PXD056356 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	LFQ DIA to analyze the proteome of NPC mutant iNeurons with and without ferric ammonium citrate
Description	Label-free quantification (LFQ) using data-independent acquisition (DIA) on the Orbitrap Astral was performed to analyze the proteome of iNeurons with NPC1 and NPC2 knocked out. The experiment includes the following time points of development: day 0, 4, 8, 16, and 22. All samples were cultured in regular media, with or without ferric ammonium citrate (FAC), and processed in triplicates for each time point, genotype, and treatment.
HostingRepository	MassIVE
AnnounceDate	2025-01-22
AnnouncementXML	Submission_2025-01-22_11:51:00.496.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yuchen He
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Astral

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-29 06:17:18	ID requested
⏵ 1	2025-01-22 11:51:00	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: proteomics, data-independent acquisition, DIA, NPC1, NPC2, iNeuron, human embryonic stem cell, hESC, ferric ammonium citrate, FAC

submitter keyword: proteomics, data-independent acquisition, DIA, NPC1, NPC2, iNeuron, human embryonic stem cell, hESC, ferric ammonium citrate, FAC

Contact List

Joshua J. Coon
contact affiliation	University of Wisconsin-Madison
contact email	jcoon@chem.wisc.edu
lab head
J. Wade Harper
contact affiliation	Harvard Medical School - Dpt of Cell Biology
contact email	wade_harper@hms.harvard.edu
lab head
Yuchen He
contact affiliation	Harvard Medical School
contact email	yuchen_he@hms.harvard.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v08/MSV000095985/

MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v08/MSV000095985/