PXD056351
PXD056351 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | AMPK-regulated glycerol excretion maintains metabolic crosstalk between reductive and energetic stress |
Description | Glucose metabolism has been studied extensively, but the role of glucose-derived excretory glycerol remains unclear. Here, we show that hypoxia induces NADH accumulation to promote glycerol excretion and this pathway consumes NADH continuously, thus attenuating its accumulation and reductive stress. Aldolase B (ALDOB) accounts for glycerol biosynthesis by forming a complex with glycerol-3-phosphate dehydrogenases, GPD1 and GPD1L. Blocking GPD1, GPD1L, or glycerol 3-phosphate phosphatase exacerbates reductive stress and suppresses cell proliferation under hypoxia and tumor growth in vivo. Over-expression of these enzymes increases glycerol excretion but still reduces cell viability under hypoxia and tumor proliferation due to energy stress. AMPK inactivates ALDOB to mitigate glycerol synthesis that dissipates ATP, alleviating NADH accumulation-induced energy crisis. Therefore, glycerol biosynthesis/excretion regulates the trade-off between reductive stress and energy stress. Moreover, this mode of regulation seems to be prevalent in reductive stress-driven transformations, enhancing our understanding of the metabolic complexity and guiding tumor treatment. |
HostingRepository | iProX |
AnnounceDate | 2024-09-29 |
AnnouncementXML | Submission_2024-09-28_20:49:56.023.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Ronghui Yang |
SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2024-09-28 20:42:01 | ID requested | |
⏵ 1 | 2024-09-28 20:49:56 | announced |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Phosphorylation sites, ALDOB, PGP |
Contact List
Binghui Li | |
---|---|
contact affiliation | Beijing Institute of Hepatology, Beijing Youan Hospital, Capital Medical University |
contact email | bli@ccmu.edu.cn |
lab head | |
Ronghui Yang | |
contact affiliation | Beijing Institute of Hepatology, Beijing Youan Hospital, Capital Medical University |
contact email | rhy_yangronghui@sina.com |
dataset submitter |
Full Dataset Link List
iProX dataset URI |