PXD056345

PXD056345 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	GingisREX: A complimentary enzyme for the detection of bacterial proteins
Description	Reliable enzymatic digestion is crucial for successful mass spectrometry-based proteomics. This study compares the arginine-specific protease, GingisREX, with a traditional trypsin/lys-C mixture for identifying E. coli proteins. Using GingisREX resulted in more protein identifications due to fewer peptides per protein, creating a simpler mixture. GingisREX also showed better digestion efficiency, fewer missed cleavages, and improved MS/MS data quality for larger peptides. These findings suggest GingisREX is a valuable complement to trypsin for detecting bacterial proteins and could be optimized as an effective alternative for identifying HCPs in biotherapeutics.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_03:23:34.603.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thomas Powell
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-28 12:57:55	ID requested
⏵ 1	2025-05-07 03:23:35	announced

Publication List

10.1021/jasms.4c00347;
Powell T, Widdowson P, N, ä, geli A, Ebner M, Creese A, GingisREX: A Complementary Enzyme for the Detection of Bacterial Proteins. J Am Soc Mass Spectrom, 35(12):2790-2794(2024) [pubmed]

10.1021/jasms.4c00347;

Powell T, Widdowson P, N, ä, geli A, Ebner M, Creese A, GingisREX: A Complementary Enzyme for the Detection of Bacterial Proteins. J Am Soc Mass Spectrom, 35(12):2790-2794(2024) [pubmed]

Keyword List

submitter keyword: LC-MS, E-coli

submitter keyword: LC-MS, E-coli

Contact List

Thomas John Powell
contact affiliation	Immunocore, Milton, UK
contact email	thomas.powell@immunocore.com
lab head
Thomas Powell
contact affiliation	Immunocore
contact email	thomas.powell@immunocore.com
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD056345
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD056345

PRIDE project URI

Repository Record List

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