PXD056334 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Molecular mechanism of ssDNA-mediated allosteric activation of the WYL-domain containing transcription regulator, DriD |
Description | WYL domain containing transcription factors play critical roles in regulating fundamental processes in bacterial physiology. The Caulobacter crescentus DriD protein is a WYL transcription regulator recently shown to activate a DNA damage response pathway that is independent of the major bacterial SOS repair system. DriD is a 327-residue homodimer and contains a unique fold composed of a N-terminal winged helix-turn-helix DNA binding domain (DNABD), linker region, three-helix bundle (3HB), WYL domain, and WYL C-terminal extension (WCX) dimerization domain. DriD binds ssDNA, which acts as a genotoxic signal that accumulates during double stranded DNA breaks, within its WYL domain. This interaction activates target DNA binding by DriD. However, the molecular mechanism by which ssDNA binding to the WYL domain, which is ~50 Å from the DNABD, activates DriD is unknown. Here, we describe structural and biochemical studies that indicate a novel allosteric mechanism of DriD activation. These data indicate that there is an inhibitory interaction formed between the DNABD and 3HB in the apo form, which is freed upon ssDNA binding. DriD represents the largest class of WYL regulator and thus can serve as a model for understanding activation by WYL activators including those in pathogenic bacteria. |
HostingRepository | PRIDE |
AnnounceDate | 2025-04-14 |
AnnouncementXML | Submission_2025-04-14_10:26:27.119.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Thomas Wales |
SpeciesList | scientific name: Caulobacter phage CcrColossus; NCBI TaxID: 1211640; |
ModificationList | No PTMs are included in the dataset |
Instrument | Synapt MS |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-09-27 11:52:25 | ID requested | |
⏵ 1 | 2025-04-14 10:26:27 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: DriD |
transcription regulator HDX-MS |
WYL domain |
hydrogen/deuterium exchange mass spectrometry |
Contact List
Thomas E. Wales |
contact affiliation | Northeastern University |
contact email | t.wales@northeastern.edu |
lab head | |
Thomas Wales |
contact affiliation | Northeastern University |
contact email | t.wales@northeastern.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/04/PXD056334 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD056334
- Label: PRIDE project
- Name: Molecular mechanism of ssDNA-mediated allosteric activation of the WYL-domain containing transcription regulator, DriD