PXD056329

PXD056329 is an original dataset announced via ProteomeXchange.

Title	Identification of Estrogen-Responsive Proteins in the Mouse Seminal Vesicle by Mass Spectrometry-Based Proteomics
Description	Although estrogenic compounds promise therapeutic potential in treating various conditions, concerns regarding their endocrine-disrupting effects have been raised Current methodologies for screening estrogenicity in rodent models are limited to the female-specific uterotrophic bioassay. Studies have reported enlargement of seminal vesicles in orchiectomized males treated with estrogens. However, identifying estrogenicity strictly through changes in wet weights is uninformative regarding the molecular mechanisms of these agents. Therefore, protein-based biomarkers can complement and improve the sensitivity of the weight-based assessments. To this end, we present a discovery-driven proteomic analysis on 17β-estradiol's effects on the seminal vesicles. We treated orchidectomized mice with the hormone for five days, and used the vehicle-treated group as control. Differentially expressed proteins in the seminal vesicles between the two groups were identified through label-free quantification using data-dependent nanoflow liquid chromatography–tandem mass spectrometry. We identified 668 total proteins, of which 133 were found to be regulated significantly by 17β-estradiol. Ingenuity Pathway Analysis® linked them to several hormone-affected pathways, including those associated with immune function such as neutrophil degranulation. The altered protein interaction networks were also related to functions, including endocrine disruption, abnormal metabolism, and therapeutic effects. Overall, we identified several potential biomarkers for estrogenicity in the mouse seminal vesicles including those not previously linked with exogenous 17β-estradiol exposure such as spondin-1
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_03:25:12.184.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD056329
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Laszlo Prokai
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-27 07:39:00	ID requested
⏵ 1	2025-05-07 03:25:13	announced

10.3390/ph17111508;

10.6019/PXD056329;

Kapic A, Zaman K, Nguyen V, Prokai-Tatrai K, Prokai L, Identification of Estrogen-Responsive Proteins in Mouse Seminal Vesicles Through Mass Spectrometry-Based Proteomics. Pharmaceuticals (Basel), 17(11):(2024) [pubmed]

submitter keyword: 7β-estradiol

male reproductive system

mouse seminal vesicle

therapeutic effects

endocrine disruption

proteomics

bioinformatics

biomarker discovery

Laszlo Prokai, Ph.D., D.Sc
contact affiliation	Professor and Robert A. Welch Chair in Biochemistry Department of Pharmacology and Neuroscience University of North Texas,Health Science Center
contact email	Laszlo.Prokai@unthsc.edu
lab head
Laszlo Prokai
contact affiliation	Department of Pharmacology & Neuroscience UNT Health Science Center Fort Worth, TX, USA
contact email	advancedms@unthsc.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD056329
     1. Label: PRIDE project
     2. Name: Identification of Estrogen-Responsive Proteins in the Mouse Seminal Vesicle by Mass Spectrometry-Based Proteomics