PXD056326

PXD056326 is an original dataset announced via ProteomeXchange.

Title	The copper-induced viable-but-non-culturable state in Cupriavidus metallidurans CH34 is characterized by distinct proteomic response patterns- Part III
Description	Copper-based materials are actively explored for their potential as antimicrobial agents. However, recent studies show that sublethal concentrations of Cu ions can induce the viable-but-non-culturable (VBNC) cell state in certain bacteria, hampering with contamination control and monitoring. Despite the growing number of omics studies investigating the VBNC state, the underlying molecular mechanisms remain largely enigmatic. Here, we determine variations in protein expression of Cupriavidus metallidurans CH34 in different phases of the Cu-induced VBNC state via quantitative LC-MS/MS analysis at multiple sample time points. With this approach, we aimed to reveal cellular adaptations triggering VBNC formation and the characteristic spontaneous recovery of culturability (resuscitation). This submission contains controls and is part of the other pride submission PXD056297.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_03:28:42.281.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD056326
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Surya Gupta
SpeciesList	scientific name: Cupriavidus metallidurans CH34; NCBI TaxID: 266264;
ModificationList	No PTMs are included in the dataset
Instrument	autoflex

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-27 05:17:55	ID requested
⏵ 1	2025-05-07 03:28:43	announced

Patocka T, Gupta S, Mastroleo F, Leys N, Matroule JY, Van Houdt R, Time-resolved proteomic profiling of Cupriavidus metallidurans CH34 in the copper-induced viable-but-nonculturable state. Metallomics, 17(3):(2025) [pubmed]

10.6019/PXD056326;

10.1093/mtomcs/mfaf007;

submitter keyword: resuscitation, metabolism,metal resistance, dormancy, copper, LC-MS/MS

Natalie Leys
contact affiliation	Head of Microbiology Unit, SCKCEN
contact email	natalie.leys@sckcen.be
lab head
Surya Gupta
contact affiliation	SCKCEN
contact email	surya.gupta@sckcen.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD056326

PRIDE project URI

• PRIDE
  1. PXD056326
     1. Label: PRIDE project
     2. Name: The copper-induced viable-but-non-culturable state in Cupriavidus metallidurans CH34 is characterized by distinct proteomic response patterns- Part III