PXD056325

PXD056325 is an original dataset announced via ProteomeXchange.

Title	Characterization of Heterorhabditis bacteriophora Response to Insect-Derived and Non-Biological Stimuli: Insights into Nematode Recovery and Released Proteins
Description	Entomopathogenic nematodes (EPNs) are gaining significant popularity as biocontrol agents due to their ability to rapidly kill insect hosts. These nematodes produce bioactive molecules known as excreted/secreted products (ESPs) that help them overcome host immune defenses. Understanding the mechanisms that trigger ESP release and their composition is crucial for unraveling the factors underlying nematode pathogenicity and enhancing their effectiveness as biocontrol agents. Leveraging the insect parasitic nematode, Heterorhabditis bacteriophora, we describe an in vitro study focused on the recovery process of infective juveniles upon contact with selected activation materials. We evaluated three host-derived materials from Galleria mellonella larvae: live larvae, frozen larvae, and heat-inactivated homogenates; and two non-insect-derived materials: filtered water and phosphate-buffered saline. While none of the tested materials resulted in 100% IJ recovery, all induced ESP release. The ESPs were collected and analyzed using liquid chromatography-tandem mass spectrometry, resulting in the identification of 372 proteins. The highest number of proteins, approximately 200, were detected in response to heat-inactivated homogenate and phosphate-buffered saline treatment. Among the identified ESPs were proteins involved in immunomodulation and protease inhibition, both crucial for overcoming host defenses. Our findings highlight the complexity of nematode-host interactions and suggest avenues for improving biocontrol strategies through a deeper understanding of nematode activation and the released spectrum of ESPs.
HostingRepository	PRIDE
AnnounceDate	2025-02-10
AnnouncementXML	Submission_2025-02-10_04:08:04.659.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pavel Roudnický
SpeciesList	scientific name: Heterorhabditis bacteriophora; NCBI TaxID: 37862;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-27 05:14:43	ID requested
⏵ 1	2025-02-10 04:08:05	announced

10.1016/J.PESTBP.2025.106318;

submitter keyword: nematode, timTOF, proteomic,Heterorhabditis

Zbynek Zdrahal
contact affiliation	Proteomics Core Facility and Research Group Proteomics, CEITEC-MU, Masaryk University, Brno, Czech Republic
contact email	zdrahal@sci.muni.cz
lab head
Pavel Roudnický
contact affiliation	Masaryk University
contact email	p.roudnicky@mail.muni.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/02/PXD056325

PRIDE project URI

• PRIDE
  1. PXD056325
     1. Label: PRIDE project
     2. Name: Characterization of Heterorhabditis bacteriophora Response to Insect-Derived and Non-Biological Stimuli: Insights into Nematode Recovery and Released Proteins