PXD056314

PXD056314 is an original dataset announced via ProteomeXchange.

Title	ChemBioID - An integrated chemical biology workflow for the investigation of proximity-induced pharmacology by the compound NVS-BPTF-A-2
Description	Modulation of protein-protein interactions with small molecules represents an emerging area of interest in drug discovery, enabling new therapeutic paradigms unprecedented with other drug classes such as catalytic enzyme inhibitors. Immunomodulatory drugs are a prime example for proximity-induced pharmacology where in the case of multiple myeloma, degradation of oncogenic transcription factors is achieved via recruitment of an E3 ligase complex. Here, we present a streamlined workflow for profiling small molecule degraders, integrating proteomics-based techniques such as proximity labelling, in-cell interaction validation assays, ubiquitination analysis and protein degradation monitoring. Application of this combined approach to a set of degraders enabled us to identify known and novel IMiD-dependent CRBN binders, thereby revealing the extended recruitment capacity of these compounds and raising important questions about potential degrader pharmacology beyond the dogma of recruitment, ubiquitination and degradation. This project is part of the IMI EUbOPEN project.
HostingRepository	PRIDE
AnnounceDate	2025-04-30
AnnouncementXML	Submission_2025-04-30_01:36:29.360.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Matthias Gstaiger
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	biotinylated residue; ubiquitination signature dipeptidyl lysine; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-27 01:21:55	ID requested
⏵ 1	2025-04-30 01:36:29	announced

Dataset with its publication pending

submitter keyword: HEK293, BioID, LC-MS/MS

Matthias Gstaiger
contact affiliation	Dr. Matthias Gstaiger ETH Zürich Institute of Molecular Systems Biology Otto-Stern-Weg 3 8093 Zürich Switzerland
contact email	gstaigem@ethz.ch
lab head
Matthias Gstaiger
contact affiliation	Institute of Molecular Systems Biology, ETH
contact email	gstaigem@ethz.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/04/PXD056314

PRIDE project URI

• PRIDE
  1. PXD056314
     1. Label: PRIDE project
     2. Name: ChemBioID - An integrated chemical biology workflow for the investigation of proximity-induced pharmacology by the compound NVS-BPTF-A-2