PXD056305
PXD056305 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Elevated NAD+ drives Sir2A-mediated GCβ deacetylation and localization for Plasmodium ookinete gliding and mosquito infection |
Description | cGMP signal-activated ookinete gliding is essential for mosquito midgut infection of Plasmodium in malaria transmission. During ookinete development, cGMP synthesizer GCβ polarizes to a unique localization “ookinete extrados site” (OES) until ookinete maturation and activates cGMP signaling for initiating parasite motility. However, the mechanism underlying GCβ translocation from cytosol to OES remains elusive. Here, we used protein proximity labeling to search the GCβ-interacting proteins in ookinetes of the rodent malaria parasite P. yoelii, and found the top hit Sir2A, a NAD+-dependent sirtuin family deacetylase. Sir2A interacts with GCβ throughout ookinete development. In mature ookinetes, Sir2A co-localizes with GCβ at OES in a mutually dependent manner. Parasites lacking Sir2A lose GCβ localization at OES, ookinete gliding, and mosquito infection, phenocopying GCβ deficiency. GCβ is acetylated at gametocytes but is deacetylated by Sir2A for OES localization at mature ookinetes. We further demonstrated that the level of NAD+, an essential co-substrate for sirtuin, increases during the ookinete development. The NAD+ at its maximal level until ookinete maturation promotes Sir2A-catalyzed GCβ deacetylation, ensuring GCβ localization at OES. This study highlights the spatiotemporal coordination of cytosolic NAD+ level and NAD+-dependent Sir2A in regulating GCβ deacetylation and dynamic localization for Plasmodium ookinete gliding. |
HostingRepository | iProX |
AnnounceDate | 2024-09-26 |
AnnouncementXML | Submission_2024-09-26_23:25:59.488.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Yang Shi |
SpeciesList | scientific name: Plasmodium yoelii yoelii 17XNL; NCBI TaxID: 352914; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2024-09-26 23:25:37 | ID requested | |
⏵ 1 | 2024-09-26 23:25:59 | announced |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Malaria, Plasmodium, Ookinete, Motility, Deacetylation |
Contact List
Jing Yuan | |
---|---|
contact affiliation | School of Life Sciences |
contact email | yuanjing@xmu.edu.cn |
lab head | |
Yang Shi | |
contact affiliation | School of Life Sciences |
contact email | 1171641534@qq.com |
dataset submitter |
Full Dataset Link List
iProX dataset URI |