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PXD056297

PXD056297 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe copper-induced viable-but-non-culturable state in Cupriavidus metallidurans CH34 is characterized by distinct proteomic response patterns- Part II
DescriptionCopper-based materials are actively explored for their potential as antimicrobial agents. However, recent studies show that sublethal concentrations of Cu ions can induce the viable-but-non-culturable (VBNC) cell state in certain bacteria, hampering with contamination control and monitoring. Despite the growing number of omics studies investigating the VBNC state, the underlying molecular mechanisms remain largely enigmatic. Here, we determine variations in protein expression of Cupriavidus metallidurans CH34 in different phases of the Cu-induced VBNC state via quantitative LC-MS/MS analysis at multiple sample time points. With this approach, we aimed to reveal cellular adaptations triggering VBNC formation and the characteristic spontaneous recovery of culturability (resuscitation). Entry into the VBNC state correlated with a widespread response to oxidative stress as well as restricted pyruvate metabolism. The expression of specific metal resistance determinants changed with Cu exposure time and culminated in strong upregulation of proteins linked to periplasmic Cu ion detoxification during the resuscitation phase. We suggest that this delayed induction of Cu resistance proteins is caused by the gradual reconstitution of cellular energy reserves through degradation of polyhydroxybutyrate granules. Furthermore, Cu-treated cells showed upregulation of several chemotaxis proteins, and increased cell motility was observed phenotypically. Our results reveal highly dynamic proteomic response patterns and contribute fundamental insights into the VBNC state, while also emphasizing the advantages of time-resolved proteomic analysis.
HostingRepositoryPRIDE
AnnounceDate2025-05-07
AnnouncementXMLSubmission_2025-05-07_03:39:19.023.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD056297
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterSurya Gupta
SpeciesList scientific name: Cupriavidus metallidurans CH34; NCBI TaxID: 266264;
ModificationListNo PTMs are included in the dataset
Instrumentautoflex
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-09-26 13:59:50ID requested
12025-05-07 03:39:19announced
Publication List
10.6019/PXD056297;
Patocka T, Gupta S, Mastroleo F, Leys N, Matroule JY, Van Houdt R, Time-resolved proteomic profiling of Cupriavidus metallidurans CH34 in the copper-induced viable-but-nonculturable state. Metallomics, 17(3):(2025) [pubmed]
10.1093/mtomcs/mfaf007;
Keyword List
submitter keyword: resuscitation, metabolism,metal resistance, dormancy, copper, LC-MS/MS
Contact List
Natalie Leys
contact affiliationhead of Microbiology Unit, SCK CEN
contact emailnatalie.leys@sckcen.be
lab head
Surya Gupta
contact affiliationSCKCEN
contact emailsurya.gupta@sckcen.be
dataset submitter
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