PXD056295

PXD056295 is an original dataset announced via ProteomeXchange.

Title	Profiling linkage-specific ubiquitin chain functions reveals a key role of K29-linked ubiquitylation in epigenome maintenance
Description	We applied a cell-based Ub replacement strategy enabling specific, conditional abrogation of each of the seven lysine-based Ub chain types in human cells to comprehensively profile global ubiquitylation changes resulting from disabling formation of individual Ub chain types. Focusing on K29-linked ubiquitylation, we found that this linkage type is enriched among chromatin-associated proteins, and that the H3K9me3 methyltransferase SUV39H1 is a prominent cellular target of this modification. We demonstrate that K29-linked ubiquitylation is essential for the proteasomal degradation of SUV39H1 despite its extensive modification by K48-linked ubiquitylation, and that K29-linked ubiquitylation of SUV39H1 is catalyzed and reversed by TRIP12 and TRABID, respectively. Importantly, preventing K29-linked ubiquitylation of SUV39H1 markedly increases H3K9me3 levels, but not other histone marks. Collectively, our Ub replacement cell line panel and datasets provide valuable resources for illuminating cellular functions of linkage-specific ubiquitylation processes and reveal a key role for K29-linked ubiquitylation in epigenome maintenance
HostingRepository	PRIDE
AnnounceDate	2025-09-18
AnnouncementXML	Submission_2025-09-18_00:26:47.972.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Moritz Voelker-Albert
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monomethylated residue; acetylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-26 08:32:18	ID requested
⏵ 1	2025-09-18 00:26:48	announced

Dataset with its publication pending

submitter keyword: Human, ubiquitylation, PTM

Niels Mailand
contact affiliation	Research Director, Professor Protein Signaling Program Novo Nordisk Foundation Center for Protein Research University of Copenhagen
contact email	niels.mailand@cpr.ku.dk
lab head
Moritz Voelker-Albert
contact affiliation	MOLEQLAR Analytics GmbH
contact email	moritz@moleqlar.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD056295
     1. Label: PRIDE project
     2. Name: Profiling linkage-specific ubiquitin chain functions reveals a key role of K29-linked ubiquitylation in epigenome maintenance