PXD056291

PXD056291 is an original dataset announced via ProteomeXchange.

Title	Maternal Immune Activation imprints translational dysregulation and differential MAP2 phosphorylation in descendant neural stem cells
Description	To undertake a comprehensive examination of the molecular mechanisms governing the alterations induced by maternal immune activation (MIA) during gestation that impacts the subsequent neurodevelopment of progeny, we have devised an in vitro model based on neural stem cells (NSCs) sourced from fetuses carried by animals subjected to Poly I:C treatment. These neural progenitors demonstrate proliferative properties and can be effectively differentiated into both neurons and glial cells. Through transcriptomic, proteomic, and phosphoproteomic analyses conducted on these cellular models, in conjunction with counterparts from control treatments, discernible shifts in the expression levels of a specific subset of proteins implicated in neuronal function were elucidated. Noteworthy is the absence of congruence between these alterations and the transcriptomic findings, suggesting a potential dysregulation at the level of protein translation. Furthermore, a discernible discrepancy in the basal phosphorylation of proteins was observed between differentiated cells from both experimental groups, particularly within proteins associated with cytoskeletal architecture and synaptic functionality, notably those belonging to the MAP family. These observed alterations were found to correlate with changes in neuronal function, particularly with respect to synaptic plasticity and the establishment of neuronal synapses, thereby reaffirming previous assertions regarding the potential impact of MAP2 function modulation via its phosphorylation state on neuronal structure and function.
HostingRepository	PRIDE
AnnounceDate	2025-02-16
AnnouncementXML	Submission_2025-02-16_10:58:09.877.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD056291
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Sandra M Martin-Guerrero
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	2-pyrrolidone-5-carboxylic acid (Gln); phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-26 06:41:17	ID requested
⏵ 1	2025-02-16 10:58:10	announced

10.6019/PXD056291;

10.1038/s41380-025-02905-5;

Mart, í, n-Guerrero SM, Mart, í, n-Esteban, é M, Lara Ord, ó, ñ, ez AJ, C, á, novas M, Mart, í, n-Oliva D, Gonz, á, lez-Maeso J, Cutillas PR, L, ó, pez-Gim, é, nez JF, Maternal immune activation imprints translational dysregulation and differential MAP2 phosphorylation in descendant neural stem cells. Mol Psychiatry, ():(2025) [pubmed]

submitter keyword: Phosphoproteomics, neuron, Neural Stem Cells, development,Proteomics, Maternal Immune Activation

Pedro R. Cutillas
contact affiliation	Centre for Cancer Genomics and Computational Biology, Barts Cancer Institute, Queen Mary University of London. EC1M6BQ, London, UK
contact email	p.cutillas@qmul.ac.uk
lab head
Sandra M Martin-Guerrero
contact affiliation	Centre for Cancer Genomics and Computational Biology, Barts Cancer Institute. Queen Mary University of London. London
contact email	s.martinguerrero@qmul.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/02/PXD056291

PRIDE project URI

• PRIDE
  1. PXD056291
     1. Label: PRIDE project
     2. Name: Maternal Immune Activation imprints translational dysregulation and differential MAP2 phosphorylation in descendant neural stem cells