PXD056290

PXD056290 is an original dataset announced via ProteomeXchange.

Title	Dysregulation of N-glycosylation by Rpn1 knockout in spermatocytes induces male infertility via endoplasmic reticulum stress in mice
Description	N-glycosylation protein modification plays a crucial regulatory role in numerous biological processes, although their contribution to male reproduction in mammals remains largely undefined. Here, we found that Ribophorin I (RPN1), a subunit of oligosaccharyltransferase complex, is indispensable for spermatogenesis in male germ cells. Germ cell-specific Rpn1 knockout results in significant inhibition of the progression of meiosis, consequently disrupting homologous chromosome pairing, meiotic recombination, and DNA double strand breaks repair during meiosis. N-glycoproteomic profiling revealed that glycosylation levels are reduced in endoplasmic reticulum-associated proteins, while functional analyses showed that Rpn1 deficiency could inhibit endoplasmic reticulum function and trigger endoplasmic reticulum stress during meiosis and increasing apoptosis levels in mice. These findings highlight the essential physiological functions of N-glycosylation modification in male spermatogenesis and expand our understanding of its role in male fertility.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_03:31:31.164.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	mingyu zhang
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	complex glycosylation
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-26 05:51:40	ID requested
⏵ 1	2025-05-07 03:31:32	announced

Zhang M, Li M, Li H, Wan Y, Yang S, Ji S, Zhang H, Liu C, Lu G, Jiang X, Liu H, knockout in spermatocytes induces male infertility via endoplasmic reticulum stress in mice. Int J Biol Sci, 21(5):2360-2379(2025) [pubmed]

10.7150/ijbs.106468;

submitter keyword: RPN1

N-glycosylation

Meiosis

Endoplasmic reticulum stress

Fertility

Mingyu Zhang
contact affiliation	Shandong University
contact email	zmyscience@163.com
lab head
mingyu zhang
contact affiliation	shandong university
contact email	zmyscience@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD056290
     1. Label: PRIDE project
     2. Name: Dysregulation of N-glycosylation by Rpn1 knockout in spermatocytes induces male infertility via endoplasmic reticulum stress in mice