PXD056248

PXD056248 is an original dataset announced via ProteomeXchange.

Title	IWS1 Stimulates Pol II Transcription Elongation In Vivo
Description	The protein IWS1 (Interacts with SPT6 1) has been implicated in various transcription-associated processes, but whether it has a direct role in RNA polymerase (Pol) II transcription remains unclear. Here, we establish a rapid depletion system for IWS1 in human cells, and use it to elucidate its function in vivo by multi-omics kinetic analysis. We show that IWS1 depletion results in a global decrease of RNA synthesis in vivo that is due to a decrease in Pol II elongation velocity and in vitro biochemical assays reveal that IWS1 directly stimulates Pol II transcription by lowering the nucleosome entry barrier. Further analyses show that H3K36me3 decreases upon IWS1 depletion although the recruitment of the histone methyltransferase SETD2 to chromatin is unaffected. We conclude that IWS1 has a direct role in Pol II transcription and is essential for maintaining normal RNA elongation, which in turn is required for normal chromatin methylation.
HostingRepository	PRIDE
AnnounceDate	2025-07-30
AnnouncementXML	Submission_2025-07-30_05:50:39.458.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yumeng Yan
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-25 09:17:14	ID requested
⏵ 1	2025-07-30 05:50:39	announced

Dataset with its publication pending

submitter keyword: transcriptional elongation, transcription, RNA Polymerase II, histone modifications, H3K36me3, SETD2,IWS1

Henning Urlaub
contact affiliation	Bioanalytical Mass Spectrometry, Max Planck Institute for Multidisciplinary Sciences, Am Faßberg 11, 37077 Göttingen, Germany
contact email	henning.urlaub@mpinat.mpg.de
lab head
Yumeng Yan
contact affiliation	Max Planck Institute for Multidisciplinary Sciences
contact email	yumeng.yan@mpinat.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD056248

PRIDE project URI

• PRIDE
  1. PXD056248
     1. Label: PRIDE project
     2. Name: IWS1 Stimulates Pol II Transcription Elongation In Vivo