PXD056243

PXD056243 is an original dataset announced via ProteomeXchange.

Title	In-depth analysis of miRNA binding sites reveals the complex response of uterine epithelium to miR-26a-5p and miR-125b-5p during early pregnancy
Description	Post-transcriptional regulation of gene expression by miRNAs likely makes significant contributions to mRNA abundance at the embryo-maternal interface. In this study, we investigated how miR-26a-5p and miR-125b-5p contribute to molecular changes occurring in the uterine luminal epithelium, which serves as the first site of signal exchange between the mother and developing embryo. To measure de novo protein synthesis after miRNA delivery to primary uterine luminal epithelial cells, we employed pulsed stable isotope labeling by amino acids (pSILAC). We found that both miRNAs alter the proteome of luminal epithelial cells, impacting numerous cellular functions, immune responses, as well as intracellular and second messenger signaling pathways. Additionally, we identified several features of miRNA-mRNA interactions that may influence the targeting efficiency of miR-26a-5p and miR-125b-5p. Overall, our study suggests a complex interaction of miR-26a-5p and miR-125b-5p with their respective targets. However, both appear to cooperatively function in modulating the cellular environment of the luminal epithelium, facilitating the morphological and molecular changes that occur during the intensive communication between the embryo and uterus at pregnancy.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_03:38:58.848.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD056243
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Piotr Zabielski
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationList	6x(13)C; methylthiolated residue; 6x(13)C; (4; monohydroxylated residue; 6x(13)C labeled L-arginine
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-25 08:08:50	ID requested
⏵ 1	2025-05-07 03:38:59	announced

Myszczynski K, Szuszkiewicz J, Krawczynski K, Sikora M, Romaniewicz M, Guzewska MM, Zabielski P, Kaczmarek MM, In-Depth Analysis of miRNA Binding Sites Reveals the Complex Response of Uterine Epithelium to miR-26a-5p and miR-125b-5p During Early Pregnancy. Mol Cell Proteomics, 24(1):100879(2025) [pubmed]

10.1016/j.mcpro.2024.100879;

10.6019/PXD056243;

submitter keyword: miRNA-mRNA interactions, pregnancy, de novo protein synthesis, uterine luminal epithelium,pulsed SILAC

Piotr Zabielski, PhD
contact affiliation	Department of Medical Biology, Medical University of Bialystok, Bialystok, Poland
contact email	piotr.zabielski@umb.edu.pl
lab head
Piotr Zabielski
contact affiliation	Medical University of Bialystok, Department of Medical Biology
contact email	piotr.zabielski@umb.edu.pl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD056243

PRIDE project URI

• PRIDE
  1. PXD056243
     1. Label: PRIDE project
     2. Name: In-depth analysis of miRNA binding sites reveals the complex response of uterine epithelium to miR-26a-5p and miR-125b-5p during early pregnancy