PXD056229

PXD056229 is an original dataset announced via ProteomeXchange.

Title	Proteomics of lipid droplet isolated from con and Sec16b knockout livers
Description	The liver plays a critical role in lipid homeostasis, where lipids are either secreted as very-low-density lipoproteins (VLDL) or stored in lipid droplets (LDs). However, the regulatory mechanisms governing these two interconnected processes remain poorly understood. Here, we demonstrate that SEC16B is crucial for regulating lipid secretion and storage within the liver. Genome-wide association studies have identified single-nucleotide polymorphisms in SEC16B to be highly associated with serum lipid levels in humans. Hepatic Sec16b deficiency decreases serum lipid levels due to impaired VLDL lipidation and secretion. SEC16B partially localizes at ER-LD contact sites and promotes LD expansion by facilitating the targeting of ER proteins to LDs. More importantly, suppression of Sec16b dramatically lowers serum lipid levels and reduces atherosclerotic lesion size in Ldlr null mice. These data reveal a novel mechanism that coordinates VLDL and LD metabolism and suggest SEC16B as a promising therapeutic target for atherosclerosis treatment.
HostingRepository	PRIDE
AnnounceDate	2026-04-23
AnnouncementXML	Submission_2026-04-23_08:18:22.448.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wei Lu
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-25 02:23:49	ID requested
⏵ 1	2026-04-23 08:18:22	announced

Dataset with its publication pending

submitter keyword: Lipid droplet, liver, mouse

Bo Wang
contact affiliation	Department of Comparative Biosciences,University of Illinois Urbana-Champaign
contact email	bowang@illinois.edu
lab head
Wei Lu
contact affiliation	University of illinois
contact email	weilu4@illinois.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD056229
     1. Label: PRIDE project
     2. Name: Proteomics of lipid droplet isolated from con and Sec16b knockout livers