PXD056206

PXD056206 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	LC-MS/MS of SETDB1 3TD after incubation with covalent ligands
Description	In order to confirm which amino acid of the 3TD of SETDB1 is covalently modified by a set of cysteine-targeting ligands, we performed LC-MS/MS on sample containing 40 uM of SETDB1 3TD with 400 uM of ligand after overnight incubation at 4 C.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_03:21:46.092.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Melanie Uguen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-24 19:58:32	ID requested
⏵ 1	2025-05-07 03:21:46	announced

Publication List

10.1038/s41467-025-57005-3;
Uguen M, Shell DJ, Silva M, Deng Y, Li F, Szewczyk MM, Yang K, Zhao Y, Stashko MA, Norris-Drouin JL, Waybright JM, Beldar S, Rectenwald JM, Mordant AL, Webb TS, Herring LE, Arrowsmith CH, Ackloo S, Gygi SP, McGinty RK, Barsyte-Lovejoy D, Liu P, Halabelian L, James LI, Pearce KH, Frye SV, Potent and selective SETDB1 covalent negative allosteric modulator reduces methyltransferase activity in cells. Nat Commun, 16(1):1905(2025) [pubmed]

10.1038/s41467-025-57005-3;

Uguen M, Shell DJ, Silva M, Deng Y, Li F, Szewczyk MM, Yang K, Zhao Y, Stashko MA, Norris-Drouin JL, Waybright JM, Beldar S, Rectenwald JM, Mordant AL, Webb TS, Herring LE, Arrowsmith CH, Ackloo S, Gygi SP, McGinty RK, Barsyte-Lovejoy D, Liu P, Halabelian L, James LI, Pearce KH, Frye SV, Potent and selective SETDB1 covalent negative allosteric modulator reduces methyltransferase activity in cells. Nat Commun, 16(1):1905(2025) [pubmed]

Keyword List

submitter keyword: SETDB1, UNC9846, UNC10016, UNC10013, 3TD, UNC10014

submitter keyword: SETDB1, UNC9846, UNC10016, UNC10013, 3TD, UNC10014

Contact List

Laura Herring
contact affiliation	UNC Metabolomics and Proteomics Core, UNC at Chapel Hill, Chapel Hill, NC, USA.
contact email	laura_herring@med.unc.edu
lab head
Melanie Uguen
contact affiliation	UNC at Chapel Hill
contact email	muguen@unc.edu
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD056206
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD056206

PRIDE project URI

Repository Record List

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