PXD056202

PXD056202 is an original dataset announced via ProteomeXchange.

Title	Global profiling of arginine reactivity and ligandability
Description	Despite the crucial biological roles of arginine, its reactivity and ligandability in the human proteome are not well-explored. In this study, we employed the activity-based protein profiling approach using phenylglyoxal-based chemical probes to globally profile arginine residues. By screening a focused library of 13 phenylglyoxal derivatives, we identified probes that offered enhanced coverage and selectivity. This led to the quantification of 4,606 arginine sites across various human cell lines. We also assessed arginine reactivity through on-beads reductive dimethylation quantitative proteomics, which highlighted a subset of hyper-reactive arginines. Competitive fragment screening via DIA-ABPP pinpointed ligandable arginines that influence protein activities, unveiling potential targets for drug discovery. We further mapped these ligandable arginines to specific protein interfaces undergoing significant changes in solvent accessible surface area after protein-protein interactions. These mappings suggested that ligandable arginines could disrupt protein-protein interactions, a hypothesis we validated through co-immunoprecipitation experiments. This study provides a comprehensive overview of arginine reactivity and introduces new insights into the functional and ligandable landscape of the human proteome, underscoring significant therapeutic potentials.
HostingRepository	iProX
AnnounceDate	2024-09-24
AnnouncementXML	Submission_2024-09-24_19:33:47.805.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yuena Wang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-24 19:32:58	ID requested
⏵ 1	2024-09-24 19:33:48	announced

Dataset with its publication pending

submitter keyword: Argnine profiling, probe, Protein-protein interaction

Gang Li
contact affiliation	Shenzhen Bay Laboratory
contact email	ligang@szbl.ac.cn
lab head
Yuena Wang
contact affiliation	Shenzhen Bay Laboratory
contact email	1160864743@qq.com
dataset submitter

iProX dataset URI