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PXD056202

PXD056202 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleGlobal profiling of arginine reactivity and ligandability
DescriptionDespite the crucial biological roles of arginine, its reactivity and ligandability in the human proteome are not well-explored. In this study, we employed the activity-based protein profiling approach using phenylglyoxal-based chemical probes to globally profile arginine residues. By screening a focused library of 13 phenylglyoxal derivatives, we identified probes that offered enhanced coverage and selectivity. This led to the quantification of 4,606 arginine sites across various human cell lines. We also assessed arginine reactivity through on-beads reductive dimethylation quantitative proteomics, which highlighted a subset of hyper-reactive arginines. Competitive fragment screening via DIA-ABPP pinpointed ligandable arginines that influence protein activities, unveiling potential targets for drug discovery. We further mapped these ligandable arginines to specific protein interfaces undergoing significant changes in solvent accessible surface area after protein-protein interactions. These mappings suggested that ligandable arginines could disrupt protein-protein interactions, a hypothesis we validated through co-immunoprecipitation experiments. This study provides a comprehensive overview of arginine reactivity and introduces new insights into the functional and ligandable landscape of the human proteome, underscoring significant therapeutic potentials.
HostingRepositoryiProX
AnnounceDate2024-09-24
AnnouncementXMLSubmission_2024-09-24_19:33:47.805.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterYuena Wang
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-09-24 19:32:58ID requested
12024-09-24 19:33:48announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Argnine profiling, probe, Protein-protein interaction
Contact List
Gang Li
contact affiliationShenzhen Bay Laboratory
contact emailligang@szbl.ac.cn
lab head
Yuena Wang
contact affiliationShenzhen Bay Laboratory
contact email1160864743@qq.com
dataset submitter
Full Dataset Link List
iProX dataset URI