PXD056185

PXD056185 is an original dataset announced via ProteomeXchange.

Title	Extracellular membrane vesicle-driven crosstalk between legume plants and rhizobia: the symbiosome space as protein trafficking interface
Description	Prokaryotes and eukaryotes secrete extracellular membrane vesicles (MVs) into the extracellular milieu to preserve and transport elevated concentrations of a given cargo across long distances. MVs encapsulate metabolites, DNA, RNA, and proteins, whose abundance and composition fluctuate depending on environmental cues. Importantly, MVs are involved in eukaryote-to-prokaryote communication owing to their ability to navigate different ecological niches and exchange molecular cargoes between the two domains. Amongst the different bacterium-host relationships, rhizobium-legume symbiosis is one of the closest known to nature. A crucial developmental stage of symbiosis is the formation of N2-fixing root nodules, which are endocytosed rhizobia -called bacteroids- confined by the plant-derived membrane. The unrestricted interface between the two membranes is the symbiosome space. To date many molecular aspects of symbiosis have been extensively studied, but the possibility of interbacterial and interdomain molecule trafficking by MVs in the symbiosome space was not questioned yet. Here we unveil intensive MV trafficking within the symbiosome interface of several rhizobium-legume dual systems by developing a robust MV isolation procedure. We analyse the MVs-encased proteomes encountered in the symbiosome space of each bacterium-host partnership uncovering both conserved and differential traits of every symbiotic system. This study opens the gates for designing MV-based biotechnological tools suitable for sustainable agriculture.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_03:04:21.145.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lothar Jaensch
SpeciesList	scientific name: Lobelia burttii subsp. burttii; NCBI TaxID: 2010885; scientific name: Rhizobium tropici CIAT 899; NCBI TaxID: 698761; scientific name: Sinorhizobium fredii HH103; NCBI TaxID: 1117943;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-24 09:47:39	ID requested
⏵ 1	2025-05-07 03:04:22	announced

10.1021/acs.jproteome.4c00444;

Ayala-Garc, í, a P, Herrero-G, ó, mez I, Jim, é, nez-Guerrero I, Otto V, Moreno-de Castro N, M, ü, sken M, J, ä, nsch L, van Ham M, Vinardell JM, L, ó, pez-Baena FJ, Ollero FJ, P, é, rez-Monta, ñ, o F, Borrero-de Acu, ñ, a JM, Extracellular Vesicle-Driven Crosstalk between Legume Plants and Rhizobia: The Peribacteroid Space of Symbiosomes as a Protein Trafficking Interface. J Proteome Res, 24(1):94-110(2025) [pubmed]

submitter keyword: symbiosome space, symbiosis, legume, rhizobium,Extracellular membrane vesicles, bacteroid

Lothar Jaensch
contact affiliation	4Cellular Proteome Research, Helmholtz Centre for Infection Research, Braunschweig, Germany
contact email	Lothar.Jaensch@Helmholtz-HZI.de
lab head
Lothar Jaensch
contact affiliation	HZI
contact email	Lothar.Jaensch@Helmholtz-HZI.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD056185

PRIDE project URI

• PRIDE
  1. PXD056185
     1. Label: PRIDE project
     2. Name: Extracellular membrane vesicle-driven crosstalk between legume plants and rhizobia: the symbiosome space as protein trafficking interface